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三种氨基酸循环延伸的家庭主体蛋白调节压力反应和Entamoeba中的酶站
Meenakshi1, Sonar Shubham Balbhim1, Shilpa Sarkar1
1Department of Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur, India.
Molecular microbiology
|July 10, 2023
概括
泰尔的家庭盒蛋白 EhHbox 调节了Entamoeba histolytica 酶站. 在E.中对其正方体EiHbox1的基因沉默侵入了受损的囊形成和活力,突出了其在分化中的作用.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 在特定的环境条件下,Entamoeba histolytica会分化为囊.
- 三种氨基酸环延伸 (TALE) 家庭主体蛋白质是细胞功能至关重要的进化保守的转录因子.
- 在E. histolytica中的A TALE主体基因 (EhHbox) 和E. invadens中的其正体基因 (EiHbox1) 在压力和细胞结合期间被上调.
研究的目的:
- 调查TALE家庭盒蛋白在Entamoeba encystation中的作用.
- 确定EhHbox的DNA结合活性和核定位.
- 评估 EiHbox1 下调对 E. invadens. 的酶站效率和基因表达的影响.
主要方法:
- 在各种压力条件下的基因识别和表达分析.
- 电泳运动转移试验 (EMSA) 来确认DNA结合.
- 基因沉默使用RNA干扰来降低 EiHbox1.
- 分析胰岛素的效率,囊的活力和关键胰岛素基因的表达.
主要成果:
- EhHbox和EiHbox1在热冲击,葡萄糖和血清饥饿期间以及在结核过程中受到上调.
- 这两种蛋白都位于核中,并与特定的DNA基因 (TGACAG,TGATTGAT) 结合.
- 降低EiHbox1的调节导致缺陷囊,降低酶站效率,并改变了基因合成酶,雅各布和杰西基因的表达.
结论:
- 塔尔 (TALE) 家庭盒蛋白被保存,并在恩塔梅巴 (Entamoeba) 中作为转录因子起作用.
- EhHbox/EiHbox1在调节酶站诱导的基因和控制Entamoeba中的分化方面发挥着关键作用.
- 这些发现提供了对控制Entamoeba encystation和生存的分子机制的见解.
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