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克罗辛抑制了由ILC2s在eosinophilic鼻聚体中产生的2型炎症反应
Xu Xiaodong1,2,3, Li Tao1,4, Liu Jianmin5,6
1The Department of Otorhinolaryngology, People's Hospital of Tibet Autonomous Region, Lhasa, Tibet, China.
克罗辛通过抑制2组先天性淋巴细胞 (ILC2s) 激活,有效地降低了eosinophilic鼻聚体的2型炎症. 这项研究强调了crocin的作用.
科学领域:
- 免疫学 免疫学 免疫学
- 耳鼻喉科 耳鼻喉科 耳鼻喉科
- 药理学 药理学是指药理学的学科.
背景情况:
- 具有鼻息肉的慢性鼻炎 (CRSwNP) 呈现出明显的1型和2型炎症模式.
- 克罗辛显示有潜力调节T助手2型细胞 (Th2) 细胞因子,包括IL-4 (IL-4),并抑制核因子kappa-B (NF-κB) 途径.
研究的目的:
- 阐明2组先天性淋巴细胞 (ILC2s) 在eosinophilic鼻中2型炎症中的作用.
- 在此背景下,评估克罗辛对由ILC2s介导的2型炎症的抑制作用.
主要方法:
- 利用免疫组织化学和免疫光学来评估转录因子表达和ILC2透在鼻息肉组织中的情况.
- 已建立的ILC2激发模型使用IL-33并用crocin治疗.
- 开发了用crocin治疗的鼻息肉的扩展模型,以分析2型炎症标志物.
主要成果:
- 异性鼻膜聚合体 (NPwEos) 呈现出增加的GATA结合蛋白-3 (GATA3) 和化学吸引剂受体同类分子,表达在T助手2型细胞 (CRTH2) 阳性细胞上,同时减少T细胞 (T-bet) 阳性细胞表达的T盒.
- 在ILC2s中,IL-33刺激增强了GATA3,CRTH2和2型细胞因子 (IL-4,IL-5,IL-13) 的表达.
- 克罗辛抑制了IL-33诱导的2型炎症在实验室ILC2培养物中和SEB诱导的炎症在NPwEos扩散物中,特别是在低度 (10μM) 中.
结论:
- 克罗辛有效地抑制由ILC2激活驱动的2型炎症.
- 低度的crocin通过抑制NF-κB激活来抑制ILC2介导的2型炎症.
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