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在使用酒厂消耗谷物生物修复过程中,碳化合物污染土壤中的细菌社区动态和相关基因

Mabel Owupele Nnadi¹, Lewis Bingle¹, Keith Thomas²

¹Faculty of Health Sciences & Wellbeing, University of Sunderland, Chester Road, Sunderland SR1 3SD, UK.

Access microbiology

|July 10, 2023

概括

酒厂消耗的谷物 (BSG) 通过促进碳化合物降解细菌和基因来增强柴油生物修复. 这项研究显示,BSG修改显著加快了污染土壤中石油碳化合物的分解.

科学领域:

环境科学环境科学
微生物学微生物学
生物修复是一种生物修复.

背景情况:

酒厂使用的谷物 (BSG) 是生物修复的潜在资源.
在BSG辅助生物修复过程中对细菌群体动态和代谢变化的理解有限.
需要对BSG在降解柴油污染土壤中的有效性进行详细评估.

研究的目的:

使用BSG修正案研究柴油污染土壤的生物修复.
分析细菌社区的转变,基因表达和代谢物变化.
评估BSG在增强生物降解方面的整体有效性.

主要方法:

用BSG修改柴油污染的土壤.
对石油碳化合物 (TPH C10-C28) 总降解的分析.
细菌殖民地形成单位 (CFU) 的量化.
对于关键降解基因 (alkB,catA,xylE) 的定量PCR (qPCR).
用于细菌社区分析的16S rRNA基因amplicon的高通量测序.

主要成果:

与未经修改的土壤相比,在BSG修改的土壤中,TPH C10-C28分量的完全降解.
在修改后的处理方法中,更高的生物降解速率常量 (k) 和增加的细菌CFU.
在BSG修改土壤中,alkB,catA和xylE基因的复制数显著增加.
BSG修正案丰富的本土碳化合物降解剂,包括Acinetobacter和Pseudomonas属.
观察到的降解化合物与已知的柴油降解途径保持一致.

结论:

BSG修正案显著提高了柴油污染土壤的生物修复.
丰富特定的细菌属 (Acinetobacter, Pseudomonas) 和关键的代谢基因有助于改善降解.
结合化学,微生物学和遗传分析的整体方法对评估生物修复疗效是有效的.

关键词:

alkB,catA,xylE 代谢基因的代谢基因细菌社区概况分析生物修复生物修复酒厂使用过的谷物.柴油代谢物的代谢物总的石油碳化合物总量

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