诺基诺耐药性不会促进菌体F13在黄金葡萄球菌中的集成或切除
Helena Leinweber1, Raphael N Sieber2, Martin S Bojer1
1Department of Veterinary and Animal Sciences, University of Copenhagen, Stigbøjlen 4, 1870 Copenhagen, Denmark.
Access microbiology
|July 10, 2023
概括
黄金葡萄球菌 (Staphylococcus aureus) 的基诺耐药性突变不会影响 ΦSa3int 原体的存在. 这些发现表明,细菌DNA陀螺酶和陀螺酶IV的变化并不负责将这些免疫逃避菌体整合到牲畜相关的MRSA菌株中.
科学领域:
- 微生物学 微生物学
- 细菌学 细菌学是一门学科.
- 病毒学 病毒学
背景情况:
- 在与人类相关的金黄色葡萄球菌中,Saint的prophages是常见的,有助于免疫逃避.
- 这些菌体通常不在牲畜相关的甲素耐药黄金色杆菌 (LA-MRSA) 中,这是由于突变的附着部位.
- 然而,LA-MRSA CC398菌株的一小部分,特别是在丹麦的养猪场,都存在 ΦSa3int 菌体.
研究的目的:
- 为了调查S. aureus CC398中基诺 (FQ) 耐药性突变是否会影响 ΦSa3int 的prophage整合.
- 为了确定细菌DNA旋转酶 (gyrA) 和拓聚酶IV (grlA) 中的突变是否影响菌体与宿主重组.
主要方法:
- 引入了FQ抗性突变 (在grlA和gyrA中) 进入S. aureus 8325-4attB,具有类似CC398的附着部位.
- 在突变菌株和野生型菌株中监测了 ΦSa3int 菌体 Φ13 的集成和释放.
主要成果:
- 在FQ耐药突变物和野生型S. aureus菌株之间,没有观察到FSa3int菌体集成或释放的显著差异.
- 存在FQ耐药性突变并没有影响菌体与细菌染色体的重组效率.
结论:
- 与FQ耐药性相关的grlA和gyrA的突变不会导致LA-MRSA CC398中ΦSa3int菌体的存在.
- 在这种特定的LA-MRSA血统中发现 ΦSa3int 菌体的机制仍需要阐明.
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