通过固态发酵,由GIO和Bacillus megaterium生产西兰酶
Samuel Adedayo Fasiku1, Mobolaji Akeem Bello1, Olubusola Ayoola Odeniyi1,2
1Department of Biological Sciences, Ajayi Crowther University, Oyo Town, Oyo State, Nigeria.
Access microbiology
|July 10, 2023
概括
这项研究使用Bacillus megaterium和Aspergillus niger GIO的农业废物固态发酵产生了西兰酶. 性预处理的玉米产生了最高的酶活性,这是进一步的特征.
科学领域:
- 生物技术是生物技术.
- 酶学 是一种酶学.
- 微生物发酵 微生物发酵
背景情况:
- 克西兰酶是分解西兰酸到西酸的关键酶,在各种行业中具有应用.
- 用农业废物生产酶是一种经济和可持续的方法.
研究的目的:
- 通过使用农业废物进行固态发酵来产生西兰酶.
- 描述从选定的微生物菌株中生成的西兰酶酶.
主要方法:
- 使用Bacillus megaterium和Aspergillus niger GIO.使用各种农业基质 (玉米,米,粉,玉米,甘包,针叶树) 的固体发酵.
- 使用性预处理的玉米 (APM) 优化发酵.
- 基于温度,pH,阴离子和表面活性剂效应的西兰酶活性的表征.
主要成果:
- 在APM上种植的Aspergillus niger GIO表现出最高的西兰酶活性 (3.18 U/ml).
- 在40°C,pH值分别为5.0和6.2时观察到A. niger GIO和B. megaterium西兰酶的最佳活性.
- 酸盐 (除) 和二硫酸盐增强了酶活性,其中SDS支持最高的产量.
结论:
- 性预处理的玉米是一种有效的基质,用于A. niger GIO和B. megaterium的高产量西兰酶生产.
- 在特定的温度,pH值和添加剂条件下,特征化的西兰酶显示出显著的活性,表明其工业潜力.
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