解码黑骨的纤维肌病局部复杂染色体重排:基因差异化,选择性扫描和卡达克纳特的蛋白质编码变化
Sagar Sharad Shinde1, Ashutosh Sharma1, Nagarjun Vijay1
1Computational Evolutionary Genomics Lab, Department of Biological Sciences, IISER Bhopal, Bhauri, Madhya Pradesh, India.
Frontiers in genetics
|July 10, 2023
概括
黑骨 (BBC) 品种具有共享的纤维肌肉病 (Fm) 部位重组,导致多颜色. 卡达克纳特BBC还在Fm位置附近展示了独特的遗传特征,表明了不同的进化适应.
科学领域:
- 动物遗传学动物遗传学
- 基因组学就是基因组学.
- 进化生物学 进化生物学
背景情况:
- 黑骨肉 (BBC) 肉因其独特的感官品质而受到重视.
- 在BBC中,黑色素多颜色与20号染色体上纤维素色 (Fm) 位点的复杂染色体重组有关,增加了内甲-3 (EDN3) 基因表达.
研究的目的:
- 通过使用长读序列测序,在Silkie品种的Fm位点解决单元类型.
- 与中国和韩国BBC品种相比,研究Kadaknath BBC的进化关系和遗传特征.
主要方法:
- 对Silkie的公开长时间读取的测序数据进行分析,以确定Fm locus的单元类型.
- 包括Kadaknath在内的各种BBC品种的全基因组重新测序.
- 鉴定和描述具有选择特征的基因组区域.
主要成果:
- 这项研究证实了"Fm_2"场景对丝的复杂染色体重组.
- 包括Kadaknath在内的所有BBC检查的品种都在Fm位点共享相同的染色体重排结点.
- 在Fm位点附近的两个近位区域 (~70Kb和~300Kb) 显示了Kadaknath中独特的选择特征,其中包含具有蛋白质编码变化的基因,特别是杀菌/增加透性的蛋白质类基因.
结论:
- 这些发现证实了BBC品种的多发色素的共同遗传基础.
- 卡达克纳特在Fm位点附近拥有独特的遗传适应,可能是由选择驱动的.
- 卡达克纳斯的杀菌/增加透性的蛋白质类基因中的蛋白质编码变化可能是由于密切的物理联系而与Fm位点的搭车结果.
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