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Updated: Jul 24, 2025

Visualizing Genetic Variants, Short Targets, and Point Mutations in the Morphological Tissue Context with an RNA In Situ Hybridization Assay
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高灵敏的近期进展In Situ混合化和在使用这些方法时需要考虑的成本和好处

Shimpei Higo1, Hirotaka Ishii1, Hitoshi Ozawa2

  • 1Department of Anatomy and Neurobiology, Graduate School of Medicine, Nippon Medical School, 1-1-5, Sendagi, Bunkyo-ku, Tokyo 113-8602, Japan.

Acta histochemica et cytochemica
|July 10, 2023
PubMed
概括

本综述涵盖了高度敏感的现场杂交 (ISH) 技术的最新进展,用于可视化细胞和组织中的核酸. 它详细介绍了研究人员使用这些改进方法的原则,特性和成本.

关键词:
Clamp 鱼类 鱼类 鱼类RNA范围的范围是RNA的范围.剑鱼是一种鱼类.杂交的连锁反应是混合的.在现场混合化.

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相关概念视频

In-situ Hybridization02:31

In-situ Hybridization

In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
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FISH - Fluorescent In-situ Hybridization02:07

FISH - Fluorescent In-situ Hybridization

Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...
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