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相关概念视频

Protein Glycosylation01:25

Protein Glycosylation

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Glycosylation, the most common post-translational modification for proteins, serves diverse functions. Adding sugars to proteins makes the proteins more resistant to proteolytic digestion. Glycosylated proteins can act as markers and receptors to promote cell-cell adhesion. Additionally, they have many essential quality control functions in the cell, such as correct protein folding and facilitating transport of misfolded proteins to the cytosol, which can be degraded.
Glycosylation occurs in...
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Conjugated Proteins02:50

Conjugated Proteins

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Simple proteins and protein complexes contain only amino acids. In contrast, many other proteins, called conjugated proteins, covalently bond with non-protein moieties.
Nucleoproteins are protein complexes that contain nucleic acids, categorized as deoxyribonucleoproteins (DNPs) or ribonucleoproteins (RNPs) respectively. The nucleosome is a typical example of a DNP where nuclear DNA is associated with histone proteins. The major antigen for the Covid-19 virus SARS-CoV is an RNP that is critical...
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Glycocalyx and its Functions01:14

Glycocalyx and its Functions

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The glycocalyx is a carbohydrate-rich, fuzzy-appearing layer on the outer surface of the cell membrane. It is highly hydrophilic, because of this it attracts large amounts of water to the cell's surface. This aids the cell's interaction with the watery environment and also helps it to obtain substances dissolved in the water. It is also important for cell identification, self/non-self determination, and embryonic development and is used in cell-to-cell attachments to form tissues.
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相关实验视频

Updated: Jul 24, 2025

Engineering Antiviral Agents via Surface Plasmon Resonance
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SARS-CoV-2尖端糖基化影响功能和中和灵敏度.

Fengwen Zhang1, Fabian Schmidt1,2, Frauke Muecksch1

  • 1Laboratory of Retrovirology, The Rockefeller University, New York, NY 10065, USA.

bioRxiv : the preprint server for biology
|July 10, 2023
PubMed
概括

SARS-CoV-2尖端蛋白的糖化对病毒感染性和免疫逃避产生影响. 虽然N343甘氨酸阻碍了中和,但COVID-19疫苗接种引起了弹性抗体.

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科学领域:

  • 病毒学 病毒学
  • 免疫学 免疫学 免疫学
  • 结构生物学 结构生物学

背景情况:

  • 病毒包膜蛋白质的糖化对病毒生物学和免疫逃避至关重要.
  • 在SARS-CoV-2尖峰 (S) 糖蛋白中,有许多N-链和O-链的糖化位点.
  • 了解糖化酶的作用是开发有效疗法和疫苗的关键.

研究的目的:

  • 为了研究单个糖化位对SARS-CoV-2 S蛋白的功能影响.
  • 评估糖化如何影响SARS-CoV-2的感染性和对中和抗体的敏感性.

主要方法:

  • 利用伪型病毒感染试验来评估SARS-CoV-2 S糖基化突变体的传染性.
  • 测试了这些突变物对来自COVID-19康复个体的单克隆抗体和多克隆抗体的敏感性.

主要成果:

  • 移除大多数单个糖化位减少了伪型病毒的感染力,通常与较低的内置尖端蛋白水平相关.
  • 在受体结合域 (RBD) 内的N343处的一种甘氨酸通过RBD特异性单克隆抗体调节中和.
  • N343甘氨酸对康复期血中聚克隆抗体的敏感性降低,这表明它在免疫逃避中发挥了作用.

结论:

  • SARS-CoV-2尖端糖化在免疫逃避中发挥着重要作用,通过降低对中和抗体的敏感性.
  • 虽然N343甘氨酸可以是中和的目标,但在康复期的个体中接种疫苗会产生抗体,抵抗这种甘氨酸的抑制作用.
  • 糖化模式是SARS-CoV-2病原和宿主免疫反应的关键决定因素.