宿主适应的Pneumocystis属的真菌病原体利用遗传区域性中间体
Ousmane H Cissé1, Shelly Curran1, H Diego Folco2
1Critical Care Medicine Department, Clinical Center, National Institutes of Health, Bethesda, Maryland, USA.
bioRxiv : the preprint server for biology
|July 10, 2023
概括
用ChIP-seq识别了真菌病原体中的中间体,揭示了独特的基因跨度结构. 这表明表观遗传机制在这些物种中定义了中间体功能.
科学领域:
- 遗传学 是一个遗传学.
- 分子生物学分子生物学
- 菌类学 菌类学是指菌类学.
背景情况:
- 中介质对于染色体分离至关重要,但其发展速度很快.
- 在宿主适应的真菌病原体中形成中心仍然没有特征.
- 肺囊菌种,哺乳动物病原体,缺乏用于基因操纵的连续培养方法.
研究的目的:
- 描述哺乳动物特异性菌病原体的中心粒体结构.阿斯科米科塔属.
- 为了研究在Pneumocystis物种中中心体特征的表观遗传基础.
主要方法:
- 异质补充来评估CENP-A的功能.
- 染色体免疫沉测序 (ChIP-seq) 用于识别中心分子.
- 从短期体外培养和受感染动物模型中分析生物体.
主要成果:
- 肺囊 CENP-A 的奥斯洛格在功能上相当于 Schizosaccharomyces pombe CENP-A.
- 在三种Pneumocystis物种的16-17个单心染色体中鉴定出独特的短区域中间体 (<10kb).
- 中介质跨越活跃的基因,缺乏保存的DNA动机/重复,CENP-C在一种物种中似乎是不可用的.
- 5甲基氨酸DNA甲基化发生,但与中间体功能无关.
结论:
- 肺囊的中间体是表观遗传特异的,缺乏保存的DNA序列.
- 由于CENP-C.的可用性,可能会发生Kinetochore重新布线.
- 即使在密切相关的,古时分离的物种中,也明显出现了快速的中间体进化.
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