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Updated: Jul 24, 2025

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遗传学和环境独特地塑造了人类免疫细胞表观基因组

Wenliang Wang¹, Manoj Hariharan¹, Wubin Ding¹

¹Genomic Analysis Laboratory, The Salk Institute for Biological Studies, 10010 N Torrey Pines Rd, La Jolla, CA 92037, USA.

bioRxiv : the preprint server for biology

|July 10, 2023

概括

环境暴露和遗传变异显著改变免疫细胞表观基因组. 这项研究揭示了这些因素如何影响DNA甲基化和染色质可访问性,从而影响免疫细胞在健康和疾病中的功能.

科学领域:

免疫学免疫学免疫学
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
基因组学就是基因组学.

背景情况:

人类免疫细胞的表观基因格局受遗传和环境因素的影响.
遗传变异与环境暴露对免疫细胞表观基因组调节的确切贡献仍然不清楚.

研究的目的:

系统地研究病原体和化学暴露以及遗传变异如何塑造免疫细胞表观基因组.
区分环境暴露和遗传因素对DNA甲基化和染色质可访问性的调节作用.

主要方法:

使用单核甲基化测序和ATAC-seq分析免疫细胞表观基因组.
识别与暴露相关的差异甲基化区域 (eDMRs) 和基因型相关的DMRs (gDMRs).
分析DNA甲基化变化与染色质可访问性之间的相关性,以及SNP与meQTLs的同位化.

主要成果:

针对特定的病原体和化学物质暴露,确定了不同的eDMR,证明了甲基组和转录因子结合的环境重塑.
在DNA甲基化变化和染色质可访问性之间观察到强烈的相关性,这表明了协调的表观遗传反应.
gDMRs优先位于基因体中,与在调节区域中丰富的eDMRs形成鲜明对比,这表明不同的调节控制.
与疾病相关的单核酸多态 (SNP) 经常与甲基化定量特征位点 (meQTLs) 配合,为疾病提供细胞类型特定的遗传见解.

结论:

遗传和环境因素复杂地相互作用,塑造免疫细胞表观基因组.
环境暴露和遗传变异对免疫细胞表观遗传状态施加不同的监管控制.
这些发现为免疫相关疾病和免疫细胞调节的遗传基础提供了新的细胞类型特异性见解.

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