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相关实验视频

Updated: Jul 24, 2025

Transpupillary Two-Photon In Vivo Imaging of the Mouse Retina
09:03

Transpupillary Two-Photon In Vivo Imaging of the Mouse Retina

Published on: February 13, 2021

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在老鼠视网膜中SYNAPTOTAGMIN-9

Chris S Mesnard1,2, Cassandra L Hays1,3, Lou E Townsend1,2

  • 1Truhlsen Eye Institute and Department of Ophthalmology and Visual Sciences, University of Nebraska Medical Center, Omaha, NE 68106, USA.

bioRxiv : the preprint server for biology
|July 10, 2023
PubMed
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赛纳普托塔格明-9 (Syt9) 存在于视网膜中,在视觉处理中发挥作用. 从棒中去除Syt9会影响视网膜信号,特别是在明亮的光线条件下.

科学领域:

  • 神经科学是一个神经科学.
  • 眼科医生 眼科 眼科
  • 分子生物学分子生物学

背景情况:

  • 赛纳普托塔格明-9 (Syt9) 是一种已知用于大脑快速突触释放的传感器.
  • 在视网膜中Syt9的表达和功能在很大程度上仍然没有特征.

研究的目的:

  • 研究Syt9在哺乳动物视网膜中的存在和功能作用.
  • 确定Syt9对棒和的视觉处理的贡献.

主要方法:

  • 生成的条件淘汰赛小鼠 (Syt9fl/fl) 与Cre-driver线 (Rho-iCre,HRGP-Cre,CMV-cre) 交叉,以消除棒,或整个动物中的Syt9.
  • 进行电网膜学 (ERG) 评估视觉功能.
  • 从单个视网膜棒细胞中记录了突触释放.

主要成果:

  • 在整个视网膜中表达Syt9.
  • 整体动物Syt9淘汰赛小鼠 (CMVSyt9) 在明亮的闪光下表现出增加的scotopicERGb波.
  • 从杆子中选择性消除Syt9 (rodSyt9CKO) 减少了scotopic和 photopicb波和振荡潜力,特别是在明亮的光线下.
  • 从 (Syt9CKO) 中消除Syt9对ERG没有显著影响.
关键词:
这是一种Synaptotagmin.一个电网红图 (electroretinogram) 是一个电网红图.视网膜 视网膜 视网膜 是一个杆光感受器细胞是一种棒光感受器细胞.

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Last Updated: Jul 24, 2025

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  • 棒中Syt9的损失并没有改变自发或引起的突触释放.

    • 结论:

    • Syt9在视网膜表达,并影响视觉处理.
    • 棒中的Syt9对于调节视觉信号至关重要,特别是在线信号集成的明亮光线条件下.
    • Syt9可以通过棒路径调节形信号的传输.