深度环氧溶剂动棒吸附提取:一种快速微提取技术,用于通过光谱光度测量来确定维生素D
Sayyed Hossein Hashemi1, Massoud Kaykhaii2, Ahmad Jamali Keikha3
1Department of Marine Chemistry, Faculty of Marine Science, Chabahar Maritime University, Chabahar, Iran.
ACS omega
|July 10, 2023
概括
一种新的深度环氧溶剂调棒吸附提取 (DES-SBSE) 方法有效提取维生素D3. 该技术提供了一种灵敏且可重复的方法,用于在各种现实世界样本中分析维生素D3.
科学领域:
- 分析化学 分析化学
- 分离科学 分离科学
背景情况:
- 维生素D3分析对于健康监测至关重要.
- 传统的提取方法可能耗时,需要大量的溶剂.
- 需要开发高效和环保的提取技术.
研究的目的:
- 为了引入一种新的微提取技术,深度欧学溶剂动棒吸附提取 (DES-SBSE).
- 为了利用疏水性深度浸泡溶剂 (hDES) 作为杆吸附提取 (SBSE) 的涂层.
- 为了有效地从真实样本中提取维生素D3,进行光谱测定.
主要方法:
- 在磁杆上制备了一种由四甲基化物和甲酸组成的新型hDES涂层.
- 微提取参数的优化使用一次变量,中央复合设计和盒子-Behnken设计.
- 用光谱测量测定提取的维生素D3.
主要成果:
- 通过DES-SBSE方法,维生素D3的低检测极限为0.08μg L-1 .
- 建立了0.51000.0μg L-1的宽线性范围.
- 证明了卓越的精度,具有日内可重复性 (<3.1%) 和日间可重复性 (<4.2%).
- 棒显示耐用性超过50次提取与良好的批次到批次可重复性 (4.5%).
结论:
- DES-SBSE是一种高效和敏感的维生素D3提取方法.
- 开发的技术为样品制备提供了一个强大的,可重复使用的替代方案.
- 这种方法对各种矩阵中维生素D3的常规分析具有前景.
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