circSnd1通过miR-485-3p/Olr1信号通路促进动脉样硬化的进展
Lin Yang1, Yuhao Lin1, Chao Wang1
1Vascular Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, 710061, Shaanxi, China.
Heliyon
|July 10, 2023
概括
这项研究确定了circSnd1/miR-485-3p/Olr1轴作为动脉样硬化发展的关键调节器. 这个轴影响炎症和脂质运输,为动脉样硬化心血管疾病提供潜在的治疗点.
科学领域:
- 分子生物学分子生物学
- 心血管研究研究心血管研究
- 遗传学 是一个遗传学.
背景情况:
- 循环RNAs (circRNAs) 参与动脉样硬化心血管疾病的发病.
- 了解竞争的内源RNA (ceRNA) 网络对于阐明动脉样硬化 (AS) 发展至关重要.
- 这项研究重点关注AS中的特定circRNA-miRNA-mRNA网络.
研究的目的:
- 在动脉样硬化中研究circRNA-miRNA-mRNA网络.
- 确定一个关键的circRNA及其在AS发展中的作用.
- 探索针对这个网络的治疗潜力.
主要方法:
- 对GEO数据集的生物信息分析以确定差异表达的circRNAs和mRNAs.
- 使用R和Cytoscape构建和可视化ceRNA网络.
- 实验验证包括双露西法酶记者测定,RNA下拉,体外功能研究,ELISA和西部涂抹.
- 在体内验证使用AS小鼠模型和病毒载体.
主要成果:
- 确定circ_0082139 (circSnd1)/miR-485-3p/Olr1轴作为一个关键的监管网络.
- 实验室研究证实了轴在调节炎症和脂质运输中的作用.
- 动物实验在体内验证了circSnd1/miR-485-3p/Olr1轴在AS发展中的参与.
结论:
- 环Snd1/miR-485-3p/Olr1轴在动脉样硬化的形成和进展中起着重要作用.
- 这个轴调节关键过程,包括炎症和脂质运输.
- 针对这一轴可能为动脉样硬化提供一种新的治疗策略.
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