由于原产地父基因的AhNSP2-B07基因表达影响花生结节
Zifan Zhao1, Yichun Wang1,2, Ze Peng3
1Agronomy Department, University of Florida, Gainesville, FL, United States.
Frontiers in plant science
|July 10, 2023
概括
花生结节涉及NSP2基因. 花生中NSP2基因的非门德尔遗传表明了源代效应,雌性雌性体内表达低,与DNA甲基化无关.
科学领域:
- 植物生物学 植物生物学
- 遗传学 遗传学 是一个
- 分子生物学分子生物学
背景情况:
- 豆类与根茎植物形成共生关系,以固定.
- 结节信号通路2 (NSP2) 基因对于这种共生至关重要.
- 花生NSP2同质体中的多态性可以导致结节损失,表现出非门德尔遗传.
研究的目的:
- 研究培养花生中NSP2基因 (N位点) 的非门德尔遗传.
- 确定基表达和印记在结节形成中的作用.
主要方法:
- 开发了自我种群来分析基因型和表型分离比率.
- 检测到异合体植物的根,卵巢和花粉中的等位基表达.
- 进行了双硫酸盐PCR和测序,以评估游戏组织中的DNA甲基化.
主要成果:
- 在共生过程中,只有一个NSP2等位基因表达在花生根中,决定结节.
- 异合体植物只有当主导等位基因被表达时才显示结节.
- 卵巢中的NSP2基因表达明显低于花粉中的NSP2,这表明雌性性子体的印记.
- 游戏组织之间没有发现显著的DNA甲基化差异.
结论:
- 花生结节受NSP2位置的原生效应的影响,特别是在雌性性质细胞中印记.
- 雌性性子体的低表达可能不是由DNA甲基化引起的.
- 这项研究揭示了一种独特的遗传基础,用于多类豆类中的共生调节.
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