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研究领域生物医学和临床科学心血管医学和血液学心脏病 (包括心血管疾病)
组织细胞外囊泡的多组学识别了动脉样硬化和动脉狭窄的独特调节者

组织细胞外囊泡的多组学识别了动脉样硬化和动脉狭窄的独特调节者

Mark C Blaser ¹, Fabrizio Buffolo ¹, Arda Halu ^1,2, Mandy E Turner ¹, Florian Schlotter ¹, Hideyuki Higashi ¹, Lorena Pantano ³, Cassandra L Clift ¹, Louis A Saddic ¹, Samantha K Atkins ¹, Maximillian A Rogers ¹, Tan Pham ¹, Amélie Vromman ², Eugenia Shvartz ², Galina K Sukhova ², Silvia Monticone ⁴, Giovanni Camussi ⁵, Simon C Robson ⁶, Simon C Body ⁷, Jochen D Muehlschlegel ⁸, Sasha A Singh ¹, Masanori Aikawa ^1,2,9, Elena Aikawa ^1,9

Mark C Blaser ¹, Fabrizio Buffolo ¹, Arda Halu ^1,2

¹Center for Interdisciplinary Cardiovascular Sciences, Cardiovascular Division, Department of Medicine (M.C.B., F.B., A.H., M.E.T., F.S., H.H., C.L.C., L.A.S., S.K.A., M.A.R., T.P., S.A.S., M.A., E.A.).
²Channing Division of Network Medicine, Department of Medicine (A.H., M.A.).
³T H Chan School of Public Health, Harvard University, Boston, MA (L.P.).
⁴Division of Internal Medicine and Hypertension (S.M.), University of Torino, Italy.
⁵Department of Medical Sciences (G.C.), University of Torino, Italy.
⁶Center for Inflammation Research, Department of Anesthesia, Critical Care and Pain Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA (S.C.R.).
⁷Boston University School of Medicine, Boston, MA (S.C.B.).
⁸Center for Perioperative Genomics, Department of Anesthesiology, Perioperative and Pain Medicine (J.D.M.), Brigham and Women's Hospital, Harvard Medical School, Boston, MA.
⁹Center for Excellence in Vascular Biology, Cardiovascular Division, Department of Medicine (A.V., E.S., G.K.S., M.A., E.A.).

⁰Center for Interdisciplinary Cardiovascular Sciences, Cardiovascular Division, Department of Medicine (M.C.B., F.B., A.H., M.E.T., F.S., H.H., C.L.C., L.A.S., S.K.A., M.A.R., T.P., S.A.S., M.A., E.A.).

Circulation +

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2023年七月10日

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在PubMed上查看摘要

概括

此摘要是机器生成的。

组织捕获的细胞外囊泡 (EVs) 在心血管化中起作用. 这项研究揭示了与动脉和门中的性信号通路相关的组织特异性电子载荷,为疾病机制提供了新的见解.

科学领域

心血管生物学
细胞外囊泡研究
蛋白质组学和转录组学

背景情况

动脉结石化和动脉样硬化通常有不同的原因.
循环细胞外囊泡 (EV) 是心血管疾病的生物标志物,但组织EV在矿化中的作用尚不清楚.

研究的目的

在心血管化中研究被组织困住的电动汽车的载荷,功能和疾病贡献.
为了比较患有动脉和主动脉的EV的蛋白质和转录形状.

主要方法

人体动脉斑块和主动脉的疾病阶段蛋白质组学.
组织EV的分离和囊泡学 (蛋白质学和小RNA测序).
在原始细胞中进行网络分析和基因验证

主要成果

冠状动脉斑块和大动脉的蛋白质表现出显著的融合,但保留了独特的蛋白质.
疾病进展改变了EV蛋白和microRNA含量,这意味着共享的途径.
组织特定的EV货物与Notch和Wnt信号联系在一起,导致化.

结论

这项研究首次比较了人体动脉斑块和主动脉的蛋白质组, 确定了不同的疾病驱动因素.
开发了一种新的囊泡学策略,用于分析纤维性组织中的EV.
组织EV在调节心血管疾病进展方面发挥着重要作用.

关键词:

大动脉狭窄症动脉样硬化细胞外囊泡微RNAs 蛋白质组学

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