评估使用单核酸多态性标记物对日本劳动马的亲属测试
Taichiro Ishige1, Mio Kikuchi1, Hironaga Kakoi1
1Genetic Analysis Department, Laboratory of Racing Chemistry, Utsunomiya, Japan.
Animal science journal = Nihon chikusan Gakkaiho
|July 10, 2023
概括
在日本,单核酸多态 (SNP) 标记物对马的血统测试是有效的. 国际动物遗传学学会 (P-ISAG) 专家小组确保了布列顿和珀切伦马的血统验证的高准确性.
科学领域:
- 动物遗传学动物遗传学
- 分子生物学分子生物学
- 基因组科学 基因组科学
背景情况:
- 亲属测试对于动物育种和遗传管理至关重要.
- 单核酸多态 (SNP) 标记器为遗传分析提供了强大的工具.
- 评估SNP在特定马品种中的实用性对于准确的遗传验证至关重要.
研究的目的:
- 评估国际动物遗传学学会 (P-ISAG) 147 SNP小组对日本布列顿 (BR) 和珀切龙 (PR) 马的亲属测试的有效性.
- 为了比较P-ISAG小组的表现与一组更大的561个SNP来发现亲属关系.
主要方法:
- 在日本从98匹马 (47 BR, 51 PR) 中提取基因组DNA.
- 使用下一代测序测序和分析P-ISAG 147个SNP面板和414个额外的自体SNP的测序.
- 计算排斥概率 (PE) 的组合概率和对母子对的评估.
主要成果:
- P-ISAG小组在两种品种中都表现出高精度的亲属验证,PEs超过0.9999.
- 在使用P-ISAG小组的35个经过验证的母子对中,没有发现排除或可疑的情况.
- 亲属发现案例显示0.18%的错误接受率,表明需要额外的标记.
结论:
- P-ISAG 147 SNP 面板在日本布列顿和珀切伦马的亲属验证方面非常有效.
- 对于在未知关系的马匹中发现亲属关系,建议使用更大的SNP集 (561个SNP),将P-ISAG面板和额外的自体标记物结合起来,以提高准确性.
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