翻译抑制剂Glorund使用可互换的RNA识别域来识别Drosophila纳米细胞
Meghan S Warden1, Eugene F DeRose2, Joel V Tamayo3
1Epigenetics and Stem Cell Biology Laboratory, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA.
Nucleic acids research
|July 10, 2023
概括
格罗伦德蛋白 (Glo) 使用多个RNA结合域来控制纳米 (nos) 翻译. 两个Glo域足以结合RNA并抑制翻译,表明它们的可互换功能.
科学领域:
- 分子生物学分子生物学
- 在RNA生物学,RNA生物学.
- 蛋白质-RNA 相互作用
背景情况:
- 众所周知,Drosophila melanogaster的蛋白质Glorund (Glo) 抑制了纳米 (nos) 的翻译.
- Glo利用其准RNA识别基因 (qRRMs) 结合G-tract和UA丰富的基因在我们的翻译控制元件 (TCE).
- 之前的研究表明多功能qRRMs,但它们在识别 nos TCE 中的联合作用尚不清楚.
研究的目的:
- 为了阐明Glo的qRRMs如何合作来识别 nos TCE.
- 确定Glo-TCE相互作用的结构基础.
- 为了研究Glo qRRMs在体内和体外足够性,用于nos翻译抑制.
主要方法:
- 一个nos TCEI_III RNA结构的溶液结构的确定.
- 在体内功能测试以评估翻译抑制.
- 进行NMR偏磁放松实验,探测Glo qRRM-RNA相互作用.
主要成果:
- 溶液结构显示,单个qRRM不能同时结合G通道和UA丰富的基因.
- 在体内实验表明,任意两个qRRM足以抑制nos翻译.
- 核磁共振和功能数据支持用于TCE识别的可互换和多功能合Glo qRRMs的模型.
结论:
- 在Glo中的多个RNA识别模块可以组合起来识别各种RNA元素.
- 串联Glo qRRMs是多功能和可互换的,用于结合G-tract或UA丰富的图案在TCE. nos中.
- 这种机制可以通过RNA结合蛋白来实现多样化的RNA识别和调节.
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