在Oryx leucoryx中识别,隔离和分子表征贝塔冠状病毒
Dan David1, Jimmy Asiku1,2, Nick Storm1
1Kimron Veterinary Institute, Beit Dagan, Israel.
Microbiology spectrum
|July 10, 2023
概括
一种新型的贝塔冠状病毒导致了阿拉伯羊的严重肠道疾病. 这项研究详细介绍了病毒.
科学领域:
- 病毒学 病毒学
- 动物传播疾病 动物传播疾病
- 动物卫生 动物健康
背景情况:
- 冠状病毒 (CoV) 是一种众所周知的多种病毒群,在哺乳动物和鸟类中引起各种疾病.
- 冠状病毒可以在物种之间传播,对公共健康构成重大威胁,如SARS-CoV-2所示.
- 野生动物的肠道疾病可能对保护工作和生态系统健康产生重大影响.
研究的目的:
- 为了隔离和表征一个冠状病毒,负责严重的肠道疾病和高死亡率在Oryx leucoryx.
- 确定新型CoV与其他已知的贝塔冠状病毒的进化关系.
- 提供第一份Oryx leucoryx.的CoV感染报告.
主要方法:
- 冠状病毒逆转录酶RT-PCR被用于初始检测.
- 电子显微镜和免疫组织化学证实了CoV的存在.
- 进行了病毒隔离,细胞培养传播 (HRT-18G) 和全基因组测序.
主要成果:
- 一种新的Betacoronavirus从患病的Oryx leucoryx中分离出来.
- 遗传学分析显示,这是一个进化上不同的Betacoronavirus,与驼冠状病毒HKU23.23密切相关.
- 该病毒属于Embecovirus亚属和Betacoronavirus 1物种. 该病毒属于Embecovirus亚属和Betacoronavirus 1物种.
结论:
- 这项研究报告了Betacoronavirus在Oryx leucoryx中引起肠道疾病的首次隔离和表征.
- 这些发现凸显了CovV在野生动物群体中出现并导致显著的发病率的潜力.
- 在动物群体中对冠状病毒的持续监测对于了解和减轻动物传播风险至关重要.
相关概念视频
01:25Rapid Identification of Pathogens
MALDI-TOF MS has transformed clinical microbiology by offering a rapid and reliable method for pathogen identification. The traditional approach to microbial identification typically involves time-consuming culture techniques and biochemical tests, which can delay the initiation of appropriate antimicrobial therapy. MALDI-TOF MS avoids these delays by using characteristic ribosomal protein mass patterns of microbial cells, enabling accurate species-level identification within minutes.Principle...
01:28Rabies
Rabies is a lethal zoonotic disease caused by a single-stranded, negative-sense RNA virus of the Lyssavirus genus, within the family Rhabdoviridae. Its primary mode of transmission to humans is through bites or saliva-contaminated scratches from infected mammals such as dogs, bats, raccoons, or foxes. Transmission can also occur if infectious saliva contacts abraded skin or intact mucous membranes, including the conjunctiva.Viral Entry and Early ReplicationOnce introduced at the bite or scratch...