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Updated: Jul 24, 2025

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5'/3'RACE方法用于测序寨卡病毒的5'和3'未翻译区域

Diego Alejandro Álvarez-Díaz1,2, José Aldemar Usme-Ciro3, Sheryll Corchuelo1

  • 1Grupo de Morfología Celular, Dirección de Investigación en Salud Pública, Instituto Nacional de Salud, Bogotá, D.C., Colombia.

Archives of virology
|July 10, 2023
PubMed
概括

寨卡病毒 (ZIKV) 的分子进化在它的RNA基因组中很明显. 一个修改后的RACE协议成功地确定了ZIKV分离物的完整的5'和3'UTR序列,有助于比较基因组学.

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RACE - Rapid Amplification of cDNA Ends02:35

RACE - Rapid Amplification of cDNA Ends

Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific...
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