道染色在可使用油的平面体的部分
Maria Rossello1,2,3, Teresa Adell4,5
1Department of Genetics, Microbiology and Statistics and Institute of Biomedicine, Universitat de Barcelona, Barcelona, Catalunya, Spain.
Methods in molecular biology (Clifton, N.J.)
|July 10, 2023
概括
这项研究提出了一个精细的协议,用于量化平面生物的亡,使用抛部分. 这种方法为研究再生和平衡提供了卓越的细胞可视化和准确性.
科学领域:
- 平面生物学的平面生物学
- 再生医学是一种再生医学.
- 细胞平衡是细胞的平衡.
背景情况:
- 平面生物是研究再生和细胞平衡的关键模型生物.
- 了解细胞可塑性需要准确量化细胞死亡和增殖.
- 目前用于分析全山平面体中亡的方法在细胞可视化方面存在局限性.
研究的目的:
- 详细介绍一项用于分析平面性抛片段中的亡细胞的协议.
- 为细胞可视化和细胞亡的量化提供更准确的方法.
- 加强对平面生物的再生和恒温的研究.
主要方法:
- 关于嵌入氨酸的平面部分的协议开发.
- 在截面上应用终端脱核样转移酶dUTP末标记 (TUNEL) 试验.
- 显微镜分析和量化组织切片中的亡细胞.
主要成果:
- 石墨烯切割协议允许详细的细胞可视化细胞亡.
- 与全山分析相比,对亡细胞的量化更准确.
- 该方法在平面再生的背景下研究细胞死亡是有效的.
结论:
- 抛切割为研究平面动物的亡提供了改进的方法.
- 该协议增强了研究再生和恒常的基础细胞机制的能力.
- 准确的亡量化对于理解平面性可塑性至关重要.
相关概念视频
IR Spectrum
When infrared (IR) radiation passes through a molecule, the bonds stretch or bend by absorbing the radiation. This absorption creates the molecule's absorption spectrum, which is the plot of its percentage transmittance versus wavenumber.
Transmittance is defined as the ratio of the radiant power passing through a sample to that from the radiation's source. Multiplying the transmittance by 100 gives the percent transmittance (%T), which varies between 100% (no absorption) and 0% (complete...
Transmittance is defined as the ratio of the radiant power passing through a sample to that from the radiation's source. Multiplying the transmittance by 100 gives the percent transmittance (%T), which varies between 100% (no absorption) and 0% (complete...
IR and UV–Vis Spectroscopy of Aldehydes and Ketones
Infrared spectroscopy, also known as vibrational spectroscopy, is mainly used to determine the types of bonds and functional groups in molecules. In aldehydes and ketones, the carbonyl (C=O) bond shows an absorption around 1710 cm-1. The C=O bond vibration of an aldehyde occurs at lower frequencies than that of a ketone. In addition to the C=O absorption in an aldehyde, the aldehydic C–H bond also gives two peaks in the 2700–2800 cm-1 range. This absorption, coupled with the C=O stretching, is...
IR and UV–Vis Spectroscopy of Carboxylic Acids
In IR spectroscopy of carboxylic acids, the C=O bond shows a characteristic band between 1710 and 1760 cm⁻¹, and the O–H bond exhibits a broad band between 2500 and 3300 cm⁻¹.
However, the stretching absorptions for the C=O bond vary depending on the structure of carboxylic acids. The C=O bond of the free carboxylic acids shows a higher stretching frequency, 1760 cm−1, while H-bonded carboxylic acids (dimers) exhibit stretching absorptions at a lower frequency, 1710 cm−1. The C=O bond of the...
However, the stretching absorptions for the C=O bond vary depending on the structure of carboxylic acids. The C=O bond of the free carboxylic acids shows a higher stretching frequency, 1760 cm−1, while H-bonded carboxylic acids (dimers) exhibit stretching absorptions at a lower frequency, 1710 cm−1. The C=O bond of the...
UV–Vis Spectroscopy of Conjugated Systems
Organic compounds with conjugated double bonds show strong absorption features in the UV–visible region of the electromagnetic spectrum attributed to π → π* electronic excitations. Generally, a UV–vis absorption spectrum is recorded as a plot of absorbance vs wavelength. The wavelength of maximum absorbance, which manifests as a peak in the absorption spectrum, is denoted as λmax.
One of the factors influencing λmax is the extent of conjugation in the...
One of the factors influencing λmax is the extent of conjugation in the...
UV–Vis Spectroscopy: Woodward–Fieser Rules
UV–Visible absorption spectra of conjugated dienes arise from the lowest energy π → π* transitions. The light-absorbing part of the molecule is called the chromophore, and the substituents directly attached to the chromophore are called auxochromes. A strong correlation exists between the absorption maxima, λmax, and the structure of a conjugated π system. The Woodward–Fieser rules predict the value of λmax for a given structure by adding the contributions...
Fixation and Sectioning
Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...


