通过使用来自大米加工和酒工业的废物进行固态发酵来生产吉布雷利克酸
Upiragibe V Pinheiro1, João H C Wancura2, Michel Brondani3
1Pro-Rectory of Infrastructure, Federal University of Santa Maria, Santa Maria, RS, Brazil.
Applied biochemistry and biotechnology
|July 10, 2023
概括
固态发酵使用原始大米和大麦麦芽残留增强的吉伯雷利克酸 (GA) 生产. 在最佳条件下,GA的产量为10.1g·kg-1,为农业增长监管机构提供了具有成本效益的替代方案.
科学领域:
- 生物技术是生物技术.
- 农业科学 农业科学
- 工业微生物学 工业微生物学
背景情况:
- 吉伯酸 (GA) 是一种重要的植物激素,用于农业中作为生长调节剂.
- 目前通过浸泡发酵的工业生产产生低度,并产生高净化成本.
- 固态发酵 (SSF) 提供了一个有前途的替代方案,用于增强GA3生产,使用具有成本效益的基材.
研究的目的:
- 调查原始大米 (RRB) 和大麦麦芽残留物 (BMR) 作为使用固态发酵 (SSF) 生产GA3基质的有效性.
- 优化SSF条件,包括水分含量,基质组成和营养补充,以最大限度地提高GA3产量.
- 在优化条件下分析GA3生产的动力学.
主要方法:
- 使用统计实验设计来评估湿度 (50-70 个重量%) 和基板组成 (30-70 个重量%) 的影响RRB/BMR比率).
- 通过分析葡萄糖 (0-80 g·L-1) 和酸 (0-5 g·L-1) 添加的影响,优化GA3的产生.
- 进行了动力学研究,以确定 GA3生产率和随时间推移的峰值产量.
主要成果:
- 最佳基质成分是30%的RRB和70%的BMR,含有70%的水分,在7天后产生最高的GA3度.
- 更高度的酸 (NH4NO3) 和中间葡萄糖水平 (40 g·L-1) 显著增强GA3的形成.
- 实现了最大GA3产量10.1g·kg的基质-1,生产在第七天达到峰值.
结论:
- 使用RRB和BMR进行固态发酵是GA3生产的有效和潜在的成本效益的方法.
- 营养物质的优化,特别是源,在最大限度地提高吉伯酸生物合成中起着至关重要的作用.
- 该研究表明,对于农业应用而言,传统的浸泡发酵是一种可行的替代方案.
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