增长激素受体激素和对抗剂:从蛋白质表达和净化到长效配方
Yue Wang1,2, Minah Kim1, Chantal Buckley1
1Liggins Institute, University of Auckland, Auckland, New Zealand.
Protein science : a publication of the Protein Society
|July 10, 2023
概括
制造复合人体生长激素 (rhGH) 和GH受体对手 (GHAs) 提出了一些挑战. 本综述涵盖了蛋白质的生产,净化和策略,如用于改善药物配方的PEGylation.
科学领域:
- 生物技术是生物技术.
- 内分泌学 在内分泌学.
- 制药科学 制药科学
背景情况:
- 重组人体生长激素 (rhGH) 和GH受体对手 (GHAs) 是治疗生长激素疾病的关键生物治疗药物.
- 当前的制造工艺在蛋白质生成和净化方面面临着重大挑战.
- 开发长效配方对于提高药物疗效和患者服药性至关重要.
研究的目的:
- 审查生产和净化rhGH和GHAs的方法.
- 总结改善这些生物治疗药物的药理动力学和药理动力学特性的策略.
- 讨论临床使用中的当前和正在开发的治疗剂.
主要方法:
- 关于重组蛋白质生产和净化技术的科学文献的综述.
- 分析配方策略,包括PEGylation和融合蛋白.
- 检查临床试验数据和监管部门对与GH相关的治疗药物的批准.
主要成果:
- 对于rhGH和GHAs,可使用各种复合蛋白表达系统和净化方法.
- PEGylation和融合蛋白质策略有效地提高了基于GH的药物的半衰期和疗效.
- 几种rhGH和GHA治疗药物已获得批准或处于临床开发阶段,表明该领域的进展.
结论:
- 对临床成功而言,高效的 rhGH 和 GHA 制造和配方至关重要.
- 像PEGylation这样的先进策略为开发有效,长效的GH疗法提供了有希望的解决方案.
- 持续的研究和开发对于优化GH相关疾病的治疗至关重要.
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