相关实验视频

Updated: Jul 24, 2025

Visualization of SARS-CoV-2 using Immuno RNA-Fluorescence In Situ Hybridization

Visualization of SARS-CoV-2 using Immuno RNA-Fluorescence In Situ Hybridization

Published on: December 23, 2020

烟雾和尖:本佐[a]皮林通过促进NR4A2诱导的ACE2和TMPRSS2表达来增强SARS-CoV-2感染

Wenbin Liu^1,2,3, Yue Zhao^1,2,3, Junyan Fan^1,2,3

¹Key Laboratory of Biosafety Defense, Ministry of Education, Shanghai, 200433, China.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

|July 10, 2023

概括

香烟烟雾中含有的酸 (benzo[a]pyrene) 通过增加ACE2和TMPRSS2.2,使SARS-CoV-2感染恶化. 这种机制涉及NR4A2上调,突出显示了COVID-19的吸烟风险,特别是在老年人中.

科学领域:

分子生物学分子生物学
病毒学病毒学
毒理学毒理学毒理学

背景情况:

香烟烟雾加剧了SARS-CoV-2感染,但机制尚不清楚.
香烟烟雾中的关键成分[a]皮林 (benzo[a]pyrene) 与病毒病原发生有关.

研究的目的:

阐明佐[a]烯促进SARS-CoV-2感染的分子机制.
调查核受体子家族4A号2 (NR4A2) 在这个过程中的作用.

主要方法:

研究了[a]皮林对肺细胞和有机体中ACE2和TMPRSS2表达的作用.
使用了SARS-CoV-2伪病毒和真实的Omicron BA.5.5.
检查了NR4A2与ACE2和TMPRSS2促进体的结合.
在老年小鼠和通过NR4A2敲击和干扰素刺激评估效果.

主要成果:

[a]二烯通过NR4A2上调ACE2和TMPRSS2的表达,增强SARS-CoV-2的进入.
这种影响独立于ACE2/TMPRSS2遗传变异.
在细胞和动物模型中观察到对SARS-CoV-2感染的敏感性增加.
随着年龄的增长,NR4A2,ACE2和TMPRSS2的表达增加,促进物甲基化减少.

结论:

[a]烯通过增强NR4A2介导的ACE2和TMPRSS2表达来促进SARS-CoV-2感染.
调查结果澄清了吸烟对COVID-19严重性的影响,并建议对包括老年人在内的弱势群体进行潜在的预防策略.

关键词:

在NR4A2中,血管氨酸转化酶 2 2 香烟香烟香烟香烟香烟香烟新冠病毒疾病2019年跨膜蛋白酶血清2 2 的作用.

更多相关视频

Author Spotlight: Exploring the Role of Inflammation in the Co-occurrence of Primary Sjogren's Syndrome and Lung Adenocarcinoma

Author Spotlight: Exploring the Role of Inflammation in the Co-occurrence of Primary Sjogren's Syndrome and Lung Adenocarcinoma

Published on: September 20, 2024

Live Imaging and Quantification of Viral Infection in K18 hACE2 Transgenic Mice Using Reporter-Expressing Recombinant SARS-CoV-2

Live Imaging and Quantification of Viral Infection in K18 hACE2 Transgenic Mice Using Reporter-Expressing Recombinant SARS-CoV-2

Published on: November 5, 2021

相关概念视频

Rous Sarcoma Virus (RSV) and Cancer

Rous Sarcoma Virus (RSV) and Cancer

Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
RSV is a retrovirus that contains two copies of a plus-strand RNA genome. Its genome consists of four main open...

Single Nucleotide Polymorphisms-SNPs

Single Nucleotide Polymorphisms-SNPs

A single nucleotide polymorphism or SNP is a single nucleotide variation at a specific genomic position in a large population. It is the most prevalent type of sequence variation found in the human genome. Point mutations that occur in more than 1% of the population qualify as SNPs. These are present once every 1000 nucleotides on an average in the human genome. Replacement of a purine with another purine (A/G) or a pyrimidine with another pyrimidine (C/T) is known as a transition. In contrast,...