Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

5.8K
Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
5.8K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

7.1K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
7.1K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

PSA-Responsive Aptamer-Based Switchable Aggregates of Ultrasmall Gold Nanoparticles.

Sensors (Basel, Switzerland)·2026
Same author

Microfluidic Assembly of Poly(glutamic acid) Nanogels Through SPAAC Click Chemistry.

Pharmaceutics·2025
Same author

Spatiotemporal Analysis of Glucagon Secretory Granule Dynamics.

Traffic (Copenhagen, Denmark)·2025
Same author

Fast assembly of 'clickable nanogels' for drug delivery.

Nanoscale advances·2025
Same author

Trajectory Analysis in Single-Particle Tracking: From Mean Squared Displacement to Machine Learning Approaches.

International journal of molecular sciences·2024
Same author

Nanogels: Recent Advances in Synthesis and Biomedical Applications.

Nanomaterials (Basel, Switzerland)·2024

相关实验视频

Updated: Jul 24, 2025

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells
11:06

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Published on: June 30, 2018

8.5K

设置多色TIRF显微镜到单个分子水平.

Chiara Schirripa Spagnolo1, Stefano Luin1,2

  • 1NEST Laboratory, Scuola Normale Superiore, Piazza San Silvestro 12, I-56127, Pisa, Italy.

Biomolecular concepts
|July 10, 2023
PubMed
概括

研究人员开发了一种多通道全内部反射光 (TIRF) 显微镜系统,用于同时进行多色单分子成像. 这种进步提高了灵敏度,并为先进的活细胞膜相互作用研究提供了指导方针.

科学领域:

  • 生物物理学的生物物理.
  • 细胞生物学 细胞生物学
  • 显微镜的使用方法

背景情况:

  • 先进的显微镜对于详细的生物机制研究至关重要.
  • 总内部反射光 (TIRF) 显微镜在细胞膜可视化方面表现出色,主要用于单色应用.
  • 现有的多色TIRF设置有限,阻碍了同时进行多道研究.

研究的目的:

  • 描述一个多通道TIRF显微镜系统的实现,用于同时进行双通道激发和检测.
  • 解决在多个道实现同时单分子检测的挑战.
  • 为多道TIRF实验提供优化光标签策略的指导方针.

主要方法:

  • 将商业单色TIRF设置修改为多通道系统.
  • 严格优化摄像头设置和最小化背景,以提高灵敏度.
  • 光标签的策略,包括探针选择,效率和反应正交.

主要成果:

  • 成功实施了同时使用双通道激发和检测TIRF显微镜系统.
  • 证明了高分子密度的应用,并对单分子水平检测的挑战进行了详细的分析.
  • 在光标签中确定了影响实验结果的关键因素.
关键词:
细胞膜的细胞膜.多通道TIRF显微镜多色光显微镜 多色光显微镜神经营养受体的神经营养受体单个分子成像技术

更多相关视频

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
11:15

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

Published on: May 30, 2016

25.3K
A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins
16:10

A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins

Published on: March 22, 2012

23.9K

相关实验视频

Last Updated: Jul 24, 2025

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells
11:06

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Published on: June 30, 2018

8.5K
A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
11:15

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

Published on: May 30, 2016

25.3K
A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins
16:10

A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins

Published on: March 22, 2012

23.9K

结论:

  • 开发的多通道TIRF系统显著提高了同时进行多色单分子成像的能力.
  • 优化设置和标签策略对于在多道TIRF实验中实现高灵敏度至关重要.
  • 这项工作为研究人员使用先进的TIRF显微镜研究活细胞膜上的相互作用机制提供了有价值的指导方针.