氧化应激反应,特别是katY基因,在Yersinia pseudotuberculosis中受到温度调节
Daniel Scheller1, Franziska Becker1, Andrea Wimbert1
1Ruhr University Bochum, Faculty of Biology and Biotechnology, Microbial Biology, Bochum, Germany.
PLoS genetics
|July 10, 2023
概括
致病细菌,如Yersinia伪结核病使用RNA温度计 (RNATs) 来控制氧化应激防御. 在katY中的一种新型RNAT显著提高了在宿主体温 (37°C) 上的保护.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 细菌病原体的产生
背景情况:
- 病原性细菌面临反应性氧物种 (ROS) 作为宿主防御机制.
- 细菌的氧化应激反应涉及5'-未翻译区域 (5'-UTRs) 的温度调节的RNA结构 (RNA温度计).
- 这些RNA温度计 (RNAT) 建议在宿主体温 (37°C) 上缓解转化抑制.
研究的目的:
- 系统地分析Yersinia伪结核病中ROS防御基因的转录和翻译调节.
- 研究RNA温度计 (RNATs) 在不同温度下的氧化应激过程中控制基因表达的作用.
- 为了确定特定的催化酶对H2O2排毒和细菌保护的贡献.
主要方法:
- 用RNA测序和定量逆转录PCR (qRT-PCR) 进行基因表达分析.
- 转化记者基因融合,酶性RNA结构探测和指纹测试用于调控机制调查.
- 催化酶突变体的表型表征和实时测量使用氧化还原敏感报告器 (roGFP2-Orp1).
主要成果:
- 四个ROS防御基因的转录在37°C上升调节.
- 在katY的翻译启动区域附近确定了一种新型,高效的RNA温度计 (RNAT),在37°C时驱动KatY诱导.
- 证实了KatA是主要的H2O2吸尘器,而Y.伪结核病在37°C时显示出更好的保护,与katY上调相关.
结论:
- 耶尔西尼亚表现出其氧化应激反应的多层调节.
- RNA温度计 (RNATs) 在宿主-病原体相互作用期间控制基因表达方面发挥着至关重要的作用.
- 在宿主体温下,由RNAT控制的katY表达对Yersinia的保护至关重要.
相关概念视频
Other Stress Responses in Bacteria
33
Bacteria have global regulatory systems that control several types of stress mechanisms. These include Pho regulon and the heat shock response, which are essential systems for environmental adaptation, such as nutrient limitation and proteotoxic stress. The Pho regulon and the heat shock response exemplify bacterial resilience, enabling rapid adaptation to fluctuating environmental conditions.Pho RegulonBacteria require phosphorus for essential cellular processes, including nucleic acid...
33
Stringent Response in E. coli
31
Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...
31
Diversity of Archaea IV
52
Hyperthermophilic archaea are a group of extremophiles thriving at temperatures above 80°C, often in hydrothermal vents and volcanic soils where conditions surpass the boiling point of water. At such temperatures, proteins, membranes, and DNA in most organisms degrade, but hyperthermophiles have evolved remarkable adaptations to maintain stability and function.Unique Cellular FeaturesHyperthermophilic membranes are composed of a monolayer of biphytanyl tetraether lipids, which resist...
52
Oxygen Requirements and Growth Patterns
161
Microorganisms exhibit diverse oxygen requirements and growth patterns driven by their metabolic strategies and environmental adaptations. Oxygen, while essential for many organisms, can also be toxic under certain conditions, shaping how microorganisms grow and survive.Oxygen Requirements of MicroorganismsMicroorganisms are classified based on their ability to use or tolerate oxygen:Obligate aerobes like Mycobacterium tuberculosis need oxygen for energy production, as it serves as the terminal...
161
Factors Influencing Microbial Growth: Temperature
68
Microorganisms display remarkable adaptations, enabling them to thrive in diverse ecological niches across a wide range of temperatures. Temperature profoundly influences microbial growth by affecting enzymatic activity, membrane fluidity, and other cellular processes.Each microorganism operates within a specific temperature range defined by three cardinal points: minimum, optimum, and maximum. Below the minimum temperature, membranes lose fluidity, halting transport processes. Above the...
68
Hyperthermophilic Bacteria
37
Domain Bacteria includes some unique hyperthermophilic species. They exhibit remarkable adaptations that enable survival in extreme environments.Thermotoga species are rod-shaped, gram-negative, non-sporulating hyperthermophiles that form a sheath-like envelope called a toga. They ferment sugars or starch, producing lactate, acetate, CO₂, and H₂, and can also grow via anaerobic respiration using H₂ and ferric iron. Found in hot springs and hydrothermal vents, over 20% of their...
37


