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相关概念视频

Flow Cytometry01:23

Flow Cytometry

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The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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相关实验视频

Updated: Jul 24, 2025

High-Dimensionality Flow Cytometry for Immune Function Analysis of Dissected Implant Tissues
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通过流式细胞计量定义iNKT细胞子组及其功能.

Marianthi Gioulbasani1, Ageliki Tsagaratou1,2,3

  • 1Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina.

Current protocols
|July 10, 2023
PubMed
概括

本研究详细介绍了分析不变天然杀手T (iNKT) 细胞子集的方法. 它涵盖了转录因子,表面标记物和细胞因子生产的表征,在体外和体内,用于免疫反应调节.

关键词:
细胞因子 细胞因子流动细胞计量是流动细胞计量的方法.在iNKT细胞中.一个子集,一个子集的子集.转录因子是一种转录因子.

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科学领域:

  • 免疫学 免疫学 免疫学
  • 细胞生物学 细胞生物学
  • 流动细胞计量 (Flow Cytometry) 是一种流动细胞计.

背景情况:

  • 不变的自然杀手T (iNKT) 细胞是重要的免疫调节者.
  • 根据转录因子和细胞因子生产,iNKT细胞可以在功能上进行细分.
  • 评估这些子集是了解免疫反应的关键.

研究的目的:

  • 描述用于表征iNKT细胞子集的可靠方法.
  • 为了使iNKT细胞功能和子集的详细分析.
  • 为ex vivo和in vivo评估提供协议.

主要方法:

  • 使用基于转录因子 (例如,PLZF,RORγt) 表达的流细胞计,对小鼠iNKT子集的表征.
  • 通过下游分子分析的表面标记物表达来识别iNKT子集 (RNA-seq, ATAC-seq, WGBS).
  • 通过体外激活 (PMA/ionomycin) 和体内激活 (α-galactosyl-ceramide) 的功能评估,然后进行细胞因子分析 (IFNγ,IL-4).

主要成果:

  • 建立了基于转录因子和表面标记物的iNKT细胞子集区分的协议.
  • 在体外和体外激活后评估iNKT细胞细胞因子生产的证明方法.
  • 提供了一种全面的方法来分析各种组织的iNKT细胞子集.

结论:

  • 描述的方法允许详细描述iNKT细胞子集的特征.
  • 这些协议有助于研究iNKT细胞在免疫调节中的功能.
  • 这些方法支持下游分子分析,以深入了解iNKT细胞生物学.