两种抗真菌醇药物通过分析逆流色谱分离,使用硫基乙烯-β-环氧二烯作为奇拉选择器
Huiyun Qiu1, Haiping Xiang1, Mengyi Wen1
1College of Pharmaceutical Science, Zhejiang University of Technology, Huzhou, 313200, China.
这项研究成功地分离了抗真菌药物Ketoconazole和Voriconazole,使用反流色谱 (CCC) 与合成的硫乙醇-β-环氧化 (SBE-β-CD) 奇拉选择器,实现了立体同位素的高纯度.
科学领域:
- 分析化学 分析化学
- 螺旋式分离 螺旋式分离
- 药用化学 医学化学
背景情况:
- 凯托可纳和沃里可纳等抗真菌药物通常存在于反体,影响其有效性和安全性.
- 对抗分离对于开发更安全,更有效的药剂至关重要.
- 反流色谱 (CCC) 提供了一种有前途的技术,用于奇拉分离.
研究的目的:
- 开发一种有效的托可纳和沃里可纳分离法.
- 在CCC中利用合成的硫乙-β-环氧化 (SBE-β-CD) 作为一种性选择体.
- 为了优化CCC条件,以高分辨率的奇拉分离亚醇抗真菌剂.
主要方法:
- 使用反流色谱 (CCC) 进行凯托可纳和沃里可纳的对抗分离.
- 开发和应用合成的硫乙-β-环极素 (SBE-β-CD) 作为一种性选择剂.
- 优化溶剂系统,SBE-β-CD度,温度和pH值.
- 使用高性能液体染色学 (HPLC) 分析等离子体纯度.
- 分子对接以研究包容复合体形成.
主要成果:
- 通过CCC取得了凯托可纳和沃里可纳的成功分离.
- 在优化条件下,沃里科纳的分离因子 (α ≥ 3.26) 和分辨率 (Rs= 1.82) 高.
- 通过HPLC测定,分离的醇立体异构体的纯度达到98.5%.
- 研究了SBE-β-CD替代度,度,温度和pH的影响.
结论:
- 合成的SBE-β-CD是基于CCC的凯托可纳和沃里可纳对抗分离的有效合选择器.
- 开发的CCC方法为分离这些重要的抗真菌药物提供了高效率和纯度.
- 分子对接提供了对性识别机制的见解.
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