一个染色体规模的基因组和Tremella fuciformisis蛋白质组草案
Yaxing Li1, Guanping Zhan2, Min Tu3
1College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China; Basic Forestry and Proteomics Research Center, Fujian Agriculture and forestry university, China.
International journal of biological macromolecules
|July 10, 2023
概括
这项研究介绍了Tremella fuciformis的第一个高质量的基因组和蛋白质组,揭示了其基因组组织和Tremella fuciformis多糖体 (TPS) 生物合成的洞察力. 这为未来的遗传研究提供了基础.
科学领域:
- 菌类学 菌类学是指菌类学.
- 基因组学就是基因组学.
- 蛋白质组学是指蛋白质组学.
背景情况:
- 形 (Tremella fuciformis) 是一个经济上重要的食用真菌.
- 高质量的基因组和蛋白质组对于理解其生物学和改善种植至关重要.
研究的目的:
- 测序和组装Tremella fuciformis的染色体规模的基因组.
- 建立第一个蛋白质组草案并确定参与多糖生物合成的基因.
- 为未来的研究提供基因组资源.
主要方法:
- 为了基因组组装,PacBio使用了HiFi和Hi-C测序技术.
- 射击枪蛋白质组学被用来识别不同生命阶段的细胞内蛋白质.
- 使用比较的转录组学和蛋白质组学来识别多糖生物合成基因.
主要成果:
- 一个染色体规模的基因组27.38 Mb被组装在高连续性和完整性 (93.1%的BUSCOs).
- 基因组注释确定了10,150个蛋白质编码基因,而蛋白质组草案确定了6,823种正规蛋白质.
- 确定了24个与Tremella fuciformis多糖体 (TPS) 生物合成相关的基因,在细胞中观察到更高的活性.
结论:
- 这项研究为Tremella fuciformis.提供了第一个全面的基因组和蛋白质组数据.
- 已识别的TPS生物合成基因为理解和操纵多糖生产提供了基础.
- 这项工作为推进T. fuciformis.生物和遗传研究建立了一个强大的平台.
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