维生素E的生物强化:通过使用代谢旁路组合来最大限度地提高油托克里诺和维生素E的总产量
Anji Reddy Konda1, Malleswari Gelli2, Connor Pedersen1
1Center for Plant Science Innovation, University of Nebraska-Lincoln, Lincoln, NE 68588, USA; Department of Biochemistry, University of Nebraska-Lincoln, Lincoln, NE 68588 USA; USA.
Metabolic engineering
|July 10, 2023
概括
研究人员通过基因修改同质化生物合成和代谢的途径,增强了卡梅林种子的维生素E (托可克罗曼醇) 生产. 这导致了前所未有的托科克罗曼醇含量,显著提高了油作物的维生素E含量.
科学领域:
- 植物生物技术 植物生物技术
- 代谢工程是代谢工程.
- 营养生物化学 营养生物化学
背景情况:
- 维生素E的托科克罗曼醇在植物中通过同质化前化合成.
- 托科特里昂醇生物合成使用格拉尼尔格拉尼尔二酸盐 (GGDP),而托科菲醇使用植物二酸盐 (PDP).
- 均化基拉尼尔基拉尼尔转移酶 (HGGT) 是托克醇合成的关键酶,也是生物强化的目标.
研究的目的:
- 为了最大限度地提高Camelina sativa种子中的托科克罗曼醇产量.
- 通过将种子特异性HGGT表达与增强的同质化生物合成和降低代谢相结合来实现这一目标.
主要方法:
- 大肠杆菌TyrA和Arabidopsis HPPD的同时表达,以增加同质化生物合成.
- 种子特异性RNA干扰 (RNAi) 抑制同质化氧酶 (HGO),减少同质化氧酶的代谢.
- 表达HGGT以增强托可克罗曼醇生产的前化.
主要成果:
- HPPD/TyrA的同时表达增加了tocochromanols的约2.5倍;HGO抑制增加了它们的约1.4倍.
- 仅仅HGGT表达就产生了约4倍的增加 (高达1400μg/g).
- 将HGGT与HPPD/TyrA和HGO抑制相结合,产生了前所未有的5000μg/g种子重量的托可克罗曼醇.
结论:
- 增加同质化剂的可用性对于最大限度地提高HGGT的产能至关重要.
- 同时增强同质酸盐生物合成和抑制其代谢,以及HGGT表达,大大提高了托可克罗曼醇的积累.
- 这种代谢工程策略为显著提高油作物的维生素E含量提供了一条途径.
关键词:
生物强化是一种生物强化.代谢工程是代谢工程.油菜油种油菜油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油种油托科克罗曼醇是一种多可克罗曼醇.托科特里昂醇 (托科特里昂醇) 是一种维生素E 维生素E 是一种维生素.更多相关视频
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