一个正调的电压指示器,用于大脑中延长的电气记录
S Wenceslao Evans1, Dong-Qing Shi2, Mariya Chavarha3
1Department of Neurobiology, Stanford University Medical Center, Stanford, CA, USA.
Nature methods
|July 10, 2023
概括
新的基因编码电压指示器 (GEVIs) 为长时间光学记录大脑电信号提供了增强的光稳定性. 这些改进的GEVI工具可以在神经科学研究中实现更清晰,更长的电压成像.
科学领域:
- 神经科学是一个神经科学.
- 生物技术是生物技术.
- 光学成像技术的成像
背景情况:
- 基因编码的电压指示器 (GEVIs) 允许光学记录神经电活动.
- 现有的GEVI在长时间成像的光稳定性方面存在局限性.
- 指标缺乏时间分辨率,无法精确捕获电信号.
研究的目的:
- 为扩展电压成像设计增强的GEVI,以提高光稳定性.
- 在标准的1和2光子显微镜中证明新型GEVI的实用性.
- 将新GEVI的性能与现有的电压和指标进行比较.
主要方法:
- 工程ASAP家族GEVIs与反向光电压关系的工程.
- 在标准的1和2光子显微镜设置中测试GEVI.
- 在生物样本中同时进行电压和成像.
主要成果:
- 开发了ASAP4b和ASAP4eGEVI,在去极化时显著增加光.
- 在小鼠中使用ASAP4e在几分钟内实现了单次试验尖峰检测.
- 在一光子和两光子照明下证明了ASAP4b和ASAP4e的成功性能.
- 与指标相比,它展示了与指标相比,用于尖端检测的优越时间分辨率.
结论:
- ASAP4b和ASAP4e GEVIs提高了光稳定性,使得更长时间的电压记录成为可能.
- 这些新型GEVI与标准的1和2光子显微镜兼容.
- 改进的GEVIs为神经科学研究提供了先进的功能,包括精确的电信号监控.
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