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相关概念视频

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The biological clock is involved in many aspects of regulating complex physiology in all animals. It was in 1935 when German zoologists, Hans Kalmus and Erwin Bünning, discovered the existence of circadian rhythm in Drosophila melanogaster. However, the internal molecular mechanisms behind the circadian clock remained a mystery until 1984, when Jeffrey C. Hall, Michael Rosbash, and Michael W. Young discovered the expression of the Per gene oscillating over a 24-hour cycle. In subsequent...
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The circadian—or biological—clock is an intrinsic, timekeeping, molecular mechanism that allows plants to coordinate physiological activities over 24-hour cycles called circadian rhythms. Photoperiodism is a collective term for the biological responses of plants to variations in the relative lengths of dark and light periods. The period of light-exposure is called the photoperiod.
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相关实验视频

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Parallel Measurement of Circadian Clock Gene Expression and Hormone Secretion in Human Primary Cell Cultures
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微流体方法用于模拟合的昼夜时钟.

Kui Han1, Yanyi Huang2

  • 1Changping Laboratory, Beijing, China.

Methods in molecular biology (Clifton, N.J.)
|July 10, 2023
PubMed
概括
此摘要是机器生成的。

一个新的微流体装置使得在单细胞水平上研究哺乳动物昼夜钟中的细胞间合. 这项技术成功地同步了纤维细胞,为中央时钟机制提供了新的见解.

关键词:
循环节的时钟是循环节的时间.图像成像是一种成像.细胞间合是细胞间的合.微流体学 微流体学一个单细胞的单细胞.

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Single-cell Resolution Fluorescence Live Imaging of Drosophila Circadian Clocks in Larval Brain Culture
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科学领域:

  • 时间生物学 时间生物学
  • 细胞生物学 细胞生物学
  • 生物物理学的生物物理.

背景情况:

  • 细胞间合在上神核 (SCN) 中对于哺乳动物的昼夜强度至关重要.
  • 现有用于研究细胞间合的体外方法会引起干扰,限制准确的分析.
  • 了解中央昼夜钟机制需要先进的体外模型.

研究的目的:

  • 开发一种微流体装置,用于对昼夜时钟细胞间合的量化单细胞分析.
  • 研究血管活性肠 (VIP) 在同步昼夜振荡中的作用.
  • 建立一个模仿中央时钟功能的新体外模型.

主要方法:

  • 设计和实施用于单细胞研究的微流体装置.
  • 工程老鼠成年纤维细胞 (MAF) 来表达VIP受体 (VPAC2).
  • 利用时钟突变的Cry1-/-MAF细胞来评估VIP诱导的细胞间合.

主要成果:

  • 经过证明的VIP诱导合足以同步工程MAF细胞中的昼夜振荡.
  • 展示了微流体平台保持强大的昼夜节律的能力.
  • 验证了平台作为重建中央时钟细胞间合的概念验证.

结论:

  • 微流体平台为研究细胞间调节网络提供了一种多功能工具.
  • 这种方法为哺乳动物昼夜钟的合机制提供了新的见解.
  • 该技术可以在表型上模仿SCN切片培养和体内小鼠的行为.