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相关概念视频

RNA-seq03:21

RNA-seq

10.1K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
10.1K
Ribosome Profiling02:24

Ribosome Profiling

3.6K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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相关实验视频

Updated: Jul 24, 2025

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues
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Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Published on: January 10, 2019

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通过scONE-Seqq进行单细胞基因组和转录组分析.

Lei Yu1, Angela Ruohao Wu2,3

  • 1Division of Life Science, The Hong Kong University of Science and Technology, Kowloon, Hong Kong, China.

Methods in molecular biology (Clifton, N.J.)
|July 10, 2023
PubMed
概括
此摘要是机器生成的。

我们开发了scONE-seq,这是一种用于同时进行单细胞转录组和基因组分析的多功能方法. 这种技术与冷组织兼容,为研究和药物发现提供了对瘤异质性的更深入的见解.

关键词:
冷的组织组织.一个单细胞的DNA-seqq.一个单细胞RNA-seqq.单细胞多组体的单细胞多组体.瘤异质性 瘤异质性

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An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing
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An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

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Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq
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Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq

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相关实验视频

Last Updated: Jul 24, 2025

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues
10:12

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Published on: January 10, 2019

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An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing
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An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

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Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq
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Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq

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科学领域:

  • 生物技术是生物技术.
  • 基因组学就是基因组学.
  • 癌症研究 癌症研究

背景情况:

  • 瘤细胞异质性在理解癌症方面存在挑战.
  • 单细胞多组技术为细胞多样性提供了新的见解.
  • 获得患者样本,特别是来自生物库的样本,对于研究至关重要.

研究的目的:

  • 引入scONE-seq,一种用于在单细胞水平上同时进行转录组和基因组分析的新方法.
  • 为了证明scONE-seq与来自生物库的冷组织样本的多功能性和兼容性.
  • 为研究应用提供 scONE-seq 实施的详细程序.

主要方法:

  • 开发了一种单管反应方法,用于同时分析单细胞/核转录组和基因组.
  • 确保scONE-seq方法与冷组织样本的兼容性.
  • 验证了测序库与Illumina和MGI平台的兼容性.

主要成果:

  • scONE-seq能够同时从单个细胞或细胞核进行转录组和基因组分析.
  • 该方法与冷组织兼容,扩大其适用于生物库样本的应用范围.
  • 生成了适合广泛使用的测序平台的测序库.

结论:

  • scONE-seq是研究瘤异质性的通用工具.
  • 该方法与冷组织的兼容性增强了它对大规模研究和药物发现的实用性.
  • scONE-seq 便于进行全面的单细胞多组体分析.