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一种类似于ETHYLENE INSENSITIVE3的截断蛋白质,GLYI,调节了棉花的衰老
Yayao Zhang1, Yihao Zang1, Jinwen Chen1
1Advanced Seed Science Institute, Plant Precision Breeding Academy, Zhejiang Provincial Key Laboratory of Crop Genetic Resources, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310012, China.
Plant physiology
|July 10, 2023
概括
棉花结增长 (GhLYI) 通过调节结相关基因20 (GhSAG20) 响应乙烯信号来加速植物衰老.
科学领域:
- 植物分子生物学 植物分子生物学
- 植物生理学 植物生理学
- 遗传学和基因组学 遗传学和基因组学
背景情况:
- 植物衰老是一个复杂的过程,由各种内部和外部信号调节.
- 乙烯 (ET) 是一种促进叶子衰老的关键植物激素.
- 乙烯不敏感3 (EIN3) 是一种主转录因子,调节与衰老相关的基因.
研究的目的:
- 为了研究一个独特的EIN3-LIKE 1 (EIL1) 基因,棉花LINT YIELD INCREASING (GhLYI) 在植物衰老中的作用.
- 阐明GHLYI在控制棉花老化途径中的调控机制.
主要方法:
- 在Arabidopsis和棉花中,GHLYI的子宫外表达和过度表达.
- 在目标下划分和标记 (CUT&Tag) 来识别GHLYI目标.
- 电泳运动转移试验 (EMSA),酵母1-杂交 (Y1H) 和双露西法酶试验以确认直接结合.
- 转录组分析和病毒诱导的基因沉默 (VIGS) 来评估基因功能.
主要成果:
- 截断EIN3蛋白的GLYI,作为衰老的积极调节者,在表达时加速叶子衰老.
- GhLYI直接与SENESCENCE-ASSOCIATED GENE 20 (SAG20) 的促进物结合,并激活其表达.
- 过度表达GHLYI会诱导与衰老相关的基因,包括SAG12,NAP/ANAC029和WRKY53.
- 抑制 GhSAG20 延迟了叶子衰老,表明其在 GhLYI 介导途径中的作用.
结论:
- GhLYI作为乙烯信号响应因子和棉花中植物衰老的关键调节器.
- 一个涉及 GhLYI 和其目标 GhSAG20 的监管模块控制着衰老过程.
- 这项研究为控制棉花衰老的分子机制提供了新的见解.
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