一种基于滴滴数字PCR的方法,用于识别和量化牛肉中的猪肉和肉衍生物
Huili Xu1, Xiaoyu Ma1, Zihong Ye1
1Zhejiang Provincial Key Laboratory of Biometrology and Inspection and Quarantine, College of Life Sciences, China Jiliang University, Hangzhou 310018, China.
Foods (Basel, Switzerland)
|July 11, 2023
概括
一种新的滴滴数字PCR (ddPCR) 方法可以准确地检测和量化牛肉中的猪肉和肉. 这种可靠的技术有助于识别伪造的牛肉产品,确保消费者信任和市场完整性.
科学领域:
- 食品科学与技术 食品科学与技术
- 分子生物学分子生物学
- 分析化学 分析化学
背景情况:
- 用比较便宜的肉类 (如猪肉和肉) 伪造牛肉是一个全球性问题,削弱了消费者的信心.
- 现有的检测肉类改方法可能缺乏常规分析所需的灵敏度或可靠性.
- 准确识别和量化造剂对于监管合规和消费者保护至关重要.
研究的目的:
- 开发和验证一种精确的滴滴数字PCR (ddPCR) 方法,用于检测牛肉中的猪和DNA.
- 建立一个定量方法来确定牛肉产品中伪造剂的质量比例.
- 为牛肉的常规分析和质量控制提供可靠的工具.
主要方法:
- 开发了一种滴滴数字PCR (ddPCR) 试验,针对猪肉和肉特有的单拷贝核基因.
- 引入了一个转移系数,以将DNA拷贝数与伪造物的质量比例相关联.
- 使用已知比例和商业牛肉产品的混合样本验证了该方法.
主要成果:
- 在ddPCR方法中,在牛肉中量化猪肉和肉的高线性,在1%到90% (w/w) 之间.
- 在猪肉和肉中实现了0.1% (w/w) 的低检测极限 (LOD) 和1% (w/w) 的量化极限 (LOQ).
- 这种方法在测试人工混合物和真实世界牛肉样本时被证明是准确和适用的.
结论:
- 开发的ddPCR方法提供了一种可靠和准确的手段,用于识别和量化牛肉中的猪和杂物.
- 这种技术在常规食品分析和质量保证协议中具有显著的实施潜力.
- 这些发现有助于维护牛肉供应链的完整性,增强消费者信任.
相关概念视频
PCR - Polymerase Chain Reaction
Overview
Real Time RT-PCR
Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
The real-time quantification of the number of amplified products is...
MALDI-TOF Mass Spectrometry
Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
Methods of Classification and Identification
Bacterial identification relies on a diverse array of techniques to classify and understand microorganisms, each tailored to uncover specific characteristics. Traditional morphological approaches, while still valuable, are limited for closely related or structurally simple organisms. Modern methods integrate biochemical, serological, genetic, and advanced molecular tools to achieve greater accuracy.Morphological and Biochemical TechniquesMorphological characteristics, such as cell shape and...
Modern Molecular Taxonomy
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
Rapid Identification of Pathogens
MALDI-TOF MS has transformed clinical microbiology by offering a rapid and reliable method for pathogen identification. The traditional approach to microbial identification typically involves time-consuming culture techniques and biochemical tests, which can delay the initiation of appropriate antimicrobial therapy. MALDI-TOF MS avoids these delays by using characteristic ribosomal protein mass patterns of microbial cells, enabling accurate species-level identification within minutes.Principle...


