人类子宫内膜干细胞在酸盐水凝中封装成卵细胞类细胞的分化
Diba Ghasemi1, Somayeh Ebrahimi-Barough1, Mohammad Hossein Nekoofar2,3
1Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
BioImpacts : BI
|July 11, 2023
概括
研究人员优化了视网膜酸 (RA) 度,以使人类子宫内膜介质介质干细胞 (hEnMSCs) 分化为类似卵细胞的细胞. 这种3D酸盐水凝方法显示出再生医学在治疗不孕症方面具有前景.
科学领域:
- 干细胞生物学 干细胞生物学
- 再生医学是一种再生医学.
- 生殖生物学 生殖生物学
背景情况:
- 人体子宫内膜介质干细胞 (hEnMSCs) 具有多个血统分化潜力.
- hEnMSCs对再生医学有前途,特别是在生殖应用中.
- 将hEnMSCs有效分化为功能性雌激素是至关重要的,但尚未完全理解.
研究的目的:
- 为了确定胚胎细胞衍生的hEnMSC分化最佳的视网膜酸 (RA) 度.
- 开发一种有效的卵细胞样细胞诱导介质.
- 评估2D和3D细胞培养系统的hEnMSC差异化.
主要方法:
- 在2D培养中优化视网膜酸 (RA) 度用于hEnMSC分化.
- 开发一种含有RA和骨形态遗传蛋白4 (BMP4) 的诱导介质.
- 在2D和3D酸盐水凝培养中对hEnMSC分化成卵细胞样细胞的评估.
主要成果:
- 10微米的RA被确定为7天后诱导胚芽细胞的最佳度.
- 酸盐水凝的特性通过风湿学和SEM分析得到证实.
- 封装细胞活力和水凝中的粘附被证明.
- 在3D培养中,10μM RA和50 ng/mL BMP4的组合增强了hEnMSC在3D培养中分化为卵细胞样细胞.
结论:
- 该研究成功地确定了hEnMSCs分化为卵细胞样细胞的最佳条件.
- 3D酸盐水凝培养增强了这种差异化过程.
- 这种使用3D酸盐水凝的体外方法显示出淋巴组织和细胞替代疗法的潜力.
相关概念视频
01:46Eukaryotic Compartmentalizations
One of the distinguishing features of eukaryotic cells is that they contain membrane-bound organelles, such as the nucleus and mitochondria, that carry out specialized functions. Since biological membranes are only selectively permeable to solutes, they help create a compartment with controlled conditions inside an organelle. These microenvironments are tailored to the organelle's specific functions and help isolate them from the surrounding cytosol.
For example, lysosomes in the animal cells...
For example, lysosomes in the animal cells...
01:41Spermatogenesis
Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male reproductive...
01:38Fertilization
During fertilization, an egg and sperm cell fuse to create a new diploid structure. In humans, the process occurs once the egg has been released from the ovary, and travels into the fallopian tubes. The process requires several key steps: 1) sperm present in the genital tract must locate the egg; 2) once there, sperm need to release enzymes to help them burrow through the protective zona pellucida of the egg; and 3) the membranes of a single sperm cell and egg must fuse, with the sperm...
01:33Cleavage and Blastulation
After a large-single-celled zygote is produced via fertilization, the process of cleavage occurs while zygotes travel through the uterine tube. Cleavage is a mitotic cell division that does not result in growth. With each round of successive cell division, daughter cells get increasingly smaller.
01:31Lysosomes
Lysosomes are membrane-enclosed spherical sacs derived from the Golgi apparatus. The most important function of the lysosome is degrading macromolecules and biological polymers that are released during membrane trafficking events such as the secretory, endocytic, autophagic, and phagocytic pathways. The degradation is carried out by several hydrolytic enzymes active in an acidic environment of the lysosomal lumen. These acid hydrolases are involved in cellular processes such as cell signaling,...
01:37Eukaryotic Compartmentalization
One of the distinguishing features of eukaryotic cells is that they contain membrane-bound organelles, such as the nucleus and mitochondria, that carry out specialized functions. Since biological membranes are only selectively permeable to solutes, they help create a compartment with controlled conditions inside an organelle. These microenvironments are tailored to the organelle's specific functions and help isolate them from the surrounding cytosol.
For example, lysosomes in the animal cells...
For example, lysosomes in the animal cells...