Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

8.2K
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
8.2K
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

68
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
68
X-ray Diffraction of Biological Samples01:10

X-ray Diffraction of Biological Samples

3.9K
X-ray diffraction or XRD is an analytical tool that utilizes X-rays to study ordered structures such as crystalline organic and inorganic samples, polycrystalline materials, proteins, carbohydrates, and drugs.
According to Bragg's law, when X-rays strike the sample positioned on a stage, the rays are  scattered by the electron clouds around the sample atoms. The  X-ray diffraction or scattering is caused by constructive interference of the X-ray waves that reflect off the internal...
3.9K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Genes Associated With Fracture Risk in Thoroughbred Horses Have Novel Roles in Osteogenesis.

Animal genetics·2026
Same author

Open surgical treatment of a 34-year-old arteriovenous fistula of the lateral plantar artery after failed coil embolization.

Journal of vascular surgery cases and innovative techniques·2026
Same author

Advancing three-dimensional tendon imaging using laboratory X-ray phase contrast techniques and refined sample preparation.

Scientific reports·2026
Same author

Antibody-mediated targeting of SOSIP HIV-1 Env to skin Langerhans cells potentiates humoral responses.

EBioMedicine·2026
Same author

Macromolecular crystallography at Elettra: current and future perspectives. Corrigendum.

Journal of synchrotron radiation·2026
Same author

Three-dimensional high-content imaging of unstained soft tissue with subcellular resolution using a laboratory-based X-ray microscope.

Proceedings of the National Academy of Sciences of the United States of America·2026

相关实验视频

Updated: Jul 23, 2025

Addressing Practical Issues in Atomic Force Microscopy-Based Micro-Indentation on Human Articular Cartilage Explants
08:06

Addressing Practical Issues in Atomic Force Microscopy-Based Micro-Indentation on Human Articular Cartilage Explants

Published on: October 28, 2022

1.0K

技术说明:使用实验室相对照X射线显微镜以亚细胞分辨率进行软骨成像.

Michela Esposito1, Alberto Astolfo1, Silvia Cipiccia1,2

  • 1Department of Medical Physics and Biomedical Engineering, University College London, London, UK.

Medical physics
|July 11, 2023
PubMed
概括
此摘要是机器生成的。

一个新的实验室X射线显微镜可以以亚细胞分辨率成像软骨组织,克服传统组织学的局限性. 这一突破使得在不需要同步电子设备的情况下,可以详细研究软骨细胞和骨关节炎.

关键词:
图像测试软骨成像细胞成像 细胞成像暗场是指一个暗场.阶段对比成像成像技术软组织成像学 软组织成像学的X射线显微镜.

更多相关视频

Visualization of Chondrocyte Intercalation and Directional Proliferation via Zebrabow Clonal Cell Analysis in the Embryonic Meckel’s Cartilage
06:40

Visualization of Chondrocyte Intercalation and Directional Proliferation via Zebrabow Clonal Cell Analysis in the Embryonic Meckel’s Cartilage

Published on: October 21, 2015

9.2K
Non-invasive 3D-Visualization with Sub-micron Resolution Using Synchrotron-X-ray-tomography
08:51

Non-invasive 3D-Visualization with Sub-micron Resolution Using Synchrotron-X-ray-tomography

Published on: May 27, 2008

13.3K

相关实验视频

Last Updated: Jul 23, 2025

Addressing Practical Issues in Atomic Force Microscopy-Based Micro-Indentation on Human Articular Cartilage Explants
08:06

Addressing Practical Issues in Atomic Force Microscopy-Based Micro-Indentation on Human Articular Cartilage Explants

Published on: October 28, 2022

1.0K
Visualization of Chondrocyte Intercalation and Directional Proliferation via Zebrabow Clonal Cell Analysis in the Embryonic Meckel’s Cartilage
06:40

Visualization of Chondrocyte Intercalation and Directional Proliferation via Zebrabow Clonal Cell Analysis in the Embryonic Meckel’s Cartilage

Published on: October 21, 2015

9.2K
Non-invasive 3D-Visualization with Sub-micron Resolution Using Synchrotron-X-ray-tomography
08:51

Non-invasive 3D-Visualization with Sub-micron Resolution Using Synchrotron-X-ray-tomography

Published on: May 27, 2008

13.3K

科学领域:

  • 生物医学成像学 生物医学成像学
  • 骨关节炎的研究研究.
  • 细胞生物学 细胞生物学

背景情况:

  • 软骨的微观成像对于骨关节炎研究至关重要.
  • 组织学提供细胞分辨率,但缺乏体积数据,可以引入文物.
  • 在历史上,细胞下软骨成像需要同步机设备.

研究的目的:

  • 为了展示一个基于实验室的X射线相对比显微镜用于亚细胞软骨成像.
  • 为了验证在软骨样本内解决细结构的能力.

主要方法:

  • 使用实验室X射线显微镜与强度调节面具.
  • 采用结构化的X射线束来检索传输,折射和暗场对比频道.
  • 影像化了ex vivo马类软骨样本,并将结果与同步电磁扫描和组织学进行了比较.

主要成果:

  • 在软骨样本中成功检测到单个软骨细胞.
  • 通过使用互补的对比道,证明了在肌肉细胞内检测亚细胞特征.
  • 达到的分辨率取决于面具光圈的宽度.

结论:

  • 这项研究为使用实验室式X射线显微镜对软骨进行亚细胞分辨率成像提供了第一个概念验证.
  • 开发的技术为基于同步仪的成像提供了一个潜在的替代方案,用于详细的软骨分析.