超的压力通过ROS和ATR信号传递诱导双细胞样细胞
Antoine Canat1, Derya Atilla1, Maria-Elena Torres-Padilla1,2
1Institute of Epigenetics and Stem Cells (IES), Helmholtz Zentrum München, München, Germany.
EMBO reports
|July 11, 2023
概括
超的压力会将小鼠胚胎干细胞 (ESC) 重编程为2细胞类细胞 (2CLC),即使经过恢复期. 这个过程涉及到活性氧物种 (ROS) 和ATR检查点路径.
科学领域:
- 干细胞生物学 干细胞生物学
- 细胞重新编程的细胞重编程.
- 机械生物学 机械生物学
背景情况:
- 小鼠胚胎干细胞 (ESCs) 呈现多能性.
- 一个罕见的亚群,二细胞样细胞 (2CLCs),类似于早期胚胎细胞.
- 经济学委员会和2CLC对环境线索的反应尚未完全理解.
研究的目的:
- 研究机械压力对ESC重新编程到2CLCs的影响.
- 阐明压力诱导的2CLC形成背后的分子机制.
主要方法:
- 对小鼠ESC暴露于高位应激.
- 测量反应性氧物种 (ROS) 水平.
- 评估ATR检查点的激活情况.
- 对2CLC诱导后应激的分析.
主要成果:
- 超的压力诱导2CLC的形成,证明了记忆效应.
- 压力导致ROS积累和ATR检查点激活在ESC中.
- 抑制ROS或ATR激活可以防止通过hyperosmotic介导的2CLC诱导.
- 对于2CLC诱导,ROS生成和ATR检查点在同一路径上运行.
结论:
- 机械应力,特别是高度应力,是ESC中2CLC重编程的强有力的诱导因素.
- 对压力的记忆反应促进了2CLC的诱导.
- 在压力诱导的2CLC重编程中,ROS-ATR通路至关重要.
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