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在Staphylococcus aureus中MepA流量中与tigecycline耐药性相关的突变

Honghao Huang^1,2,3, Peng Wan^1,2,3, Xinyue Luo^1,2,3

¹Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, South China Agricultural University, Guangzhou, China.

Microbiology spectrum

|July 11, 2023

概括

在MepA流量中的突变促进了黄金葡萄球菌的tigecycline耐药性,即使没有过度表达. 这项研究确定了赋予耐药性的特定MepA突变,为检测tigecycline-resistant S. aureus (TRSA) 提供了基因型标记.

科学领域:

微生物学微生物学
分子生物学分子生物学
抗微生物耐药性抗微生物耐药性

背景情况:

提格环素对于治疗多抗药性细菌感染至关重要.
抗环素黄金葡萄球菌 (TRSA) 是一个新兴的临床问题.
编码MepA流量的mepA基因与tigecycline耐药性有关,通常通过过度表达.

研究的目的:

调查mepA基因内特定突变在S. aureus中对tigecycline耐药性的作用.
评估已识别的MepA突变对tigecycline流量和药物结合的影响.
为识别TRSA菌株提供基因型参考.

主要方法:

来自各种来源的mepA基因突变的探索.
通过突变删除,补充,tigecycline积累试验和分子对接来评估已识别的突变 (T29I,E287G,T29I+E287G).
评估最小抑制度 (MIC) 和排泄抑制剂 (例如CCCP) 的作用.

主要成果:

在MepA中确定了T29I,E287G和T29I+E287G的特定突变,它们与tigecycline耐药性有关.
这些突变被证明可以促进tigecycline流量并降低结合能量,增强耐药性.
在具有这些突变的变形体中观察到tigecycline,gentamicin和amycacin的MIC增加.

结论:

在MepA流量的突变赋予S. aureus的tigecycline耐药性,独立于过度表达.
鉴定到的MepA突变可以作为检测TRSA的潜在基因型标记.
需要进一步的研究和制策略,以解决这些突变在临床环境中的潜在流行问题.

关键词:

黄金葡萄球菌黄金葡萄球菌抗生素耐药性抗生素耐药性废气排放是一种排放.排放的排放.这就是MepA的意思.这种药物是tigecycline.

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