基因型,DHA和弗拉瓦诺尔干预对老年转基因小鼠大脑DHA和脂质组特征的影响
Anneloes Martinsen1, Rasha N M Saleh1,2, Raphael Chouinard-Watkins3
1Norwich Medical School, University of East Anglia, Norwich NR4 7TJ, UK.
Nutrients
|July 11, 2023
概括
这项研究研究了醇和鱼油如何影响不同APOE基因型的小鼠的DHA水平. 黄醇对大脑的DHA状态显示出适度的益处,主要是在APOE3载体中,而不是APOE4载体.
科学领域:
- 神经科学是一个神经科学.
- 营养科学 营养科学
- 遗传学 是一个遗传学.
背景情况:
- 阿波利波蛋白E4 (APOE4) 基因型是阿尔茨海默病 (AD) 的风险因素.
- 多可萨赫萨酸 (DHA) 是一种omega-3多不和脂肪酸 (n3-PUFA),对大脑健康至关重要,但其代谢在APOE4载体中受损.
- 黄醇可能会影响DHA水平,但它们与鱼油对大脑DHA状态的联合作用,特别是通过APOE基因型,仍然不清楚.
研究的目的:
- 研究可可黄 (FLAV) 和APOE基因型对血液和大脑中的多不和脂肪酸 (PUFA) 状态的相互作用作用.
- 在小鼠模型中,确定与鱼油 (FO) 共同补充黄醇对大脑多可萨赫萨酸 (DHA) 吸收,状态和分区的影响.
主要方法:
- 使用人性化的APOE3和APOE4向替代转基因小鼠模型.
- 服用高脂肪 (HF) 饮食,富含鱼油 (FO) 和可可黄 (FLAV) 的 DHA.
- 分析了血液中的PUFA水平,红细胞 (RBC) 和特定的大脑脂质部分 (脂胆[PC],脂乙醇[PE]).
主要成果:
- 在服用鱼油补充剂后,血液和大脑中的DHA水平显著增加.
- 与APOE3小鼠相比,APOE4小鼠在补充FO和FLAV时显示出较高的红细胞 (RBC) DHA水平.
- 虽然鱼油本身并没有增加APOE4小鼠的大脑DHA百分比,但与对照组相比,特定的大脑脂质分数 (PC和PE) 在补充FO和FLAV时显示出更高的DHA水平.
- 添加弗拉瓦诺没有显著增加大脑DHA总百分比,但在APOE3小鼠中改善了大脑:RBC DHA比率.
结论:
- 可可黄醇仅对大脑的DHA状态产生适度的影响.
- 这些有益影响似乎仅限于具有APOE3基因型的个体.
- 需要进一步的研究来了解这些基因型特异性反应背后的机制.
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