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Nrf2-中介途径被Prunus spinosa激活L. (科) 水果提取物:生物信息学分析和实验验证

Mariastella Colomba¹, Serena Benedetti¹, Daniele Fraternale¹

¹Department of Biomolecular Sciences (DiSB), University of Urbino Carlo Bo, 61029 Urbino, Italy.

|July 11, 2023

概括

普鲁努斯·斯皮诺萨 (Prunus spinosa) 果实提取物通过调节Nrf2和减少炎症条件下的氧化应激,显示出抗氧化特性. 这项研究揭示了通过细胞衰老途径的潜在机制.

科学领域:

植物化学植物化学
分子生物学分子生物学
生物信息学是一种生物信息学.

背景情况:

之前的研究表明,Prunus spinosa水果 (PSF) 乙醇提取物具有抗氧化,抗微生物,抗炎和伤口愈合的特性.
基于PSF抗氧化作用的精确生物机制,特别是在促炎性托尔类受体4 (TLR4) 攻击期间,需要阐明.

研究的目的:

通过综合生物信息学和实验验证,研究PSF抗氧化活性的潜在生物机制.
在TLR4诱导的炎症反应期间,通过PSF提取物调节的关键途径和标.

主要方法:

生物信息学分析使用miRNet 2.0来预测生物过程.
化学用于识别核受体 (NR) 信号和应激反应 (SR) 途径内的目标.
在试验室实验验证中,使用人类单细胞U937细胞系与脂多糖 (LPS) 挑战.

主要成果:

miRNet分析表明,PSF提取物主要激活细胞衰老.
化学预测了抗氧化剂反应元素 (ARE) 信号,线粒体膜潜能 (MMP) 和p53 SR通路的调节.
实验验证证了PSF提取物对Nrf2的调制,导致LPS治疗的U937细胞中氧化应激和活性氧物种 (ROS) 的减少;没有观察到对p53或MMP的显著影响.

结论:

普鲁努斯·斯皮诺萨 (Prunus spinosa) 果实提取物通过调节Nrf2通路表现出抗氧化作用,从而减少氧化应激.
这些发现表明,PSF的有益作用涉及到细胞衰老的激活和特定应激反应通路的调节.

关键词:

化工制品的使用方法抗氧化剂活性抗氧化剂活性一个黑色的棘.miRNet miRNet 是一个互联网网络.氧化应激是一种氧化应激.类化合物类化合物类化合物

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