了解土壤微生物生态中的病毒影响,通过传染性细菌菌体的持久性和衰变
Alessandra G DiPietro1, Shawn A Bryant1, Matthew M Zanger1
1Biology Department, The College of William and Mary, 3037 Integrated Science Center, Williamsburg, VA, 23185, USA.
Current microbiology
|July 11, 2023
概括
测量土壤菌体衰变率是独立于宿主. 土壤菌体的衰变速度比水生菌体慢,这表明营收周转率较低,并影响土壤微生物群落.
科学领域:
- 环境微生物学环境微生物学
- 病毒学 病毒学
背景情况:
- 海洋菌体生态学得到了充分的研究,包括衰变率和宿主相互作用.
- 土壤菌体生态学,特别是衰变速度和种群动态,被理解得少得多.
研究的目的:
- 为了确定土壤和水环境中的菌体衰变率,独立于宿主相互作用.
- 为了比较土壤菌体的衰变速度与海洋和淡水菌体的衰变速度.
主要方法:
- 使用无菌土壤和水生微观世界,用单个菌体隔离物接种.
- 测量了5个模型菌体隔离物的菌体衰变率 (随时间的流逝而失去的传染性).
- 在土壤和水生微观宇宙中化菌体,以比较衰变动态.
主要成果:
- 菌体衰变速率有很大的差异,在土壤中为每小时0.11-2.07%,在水中微观宇宙中为每小时0.07-0.28%.
- 观察到的衰变速率在土壤微观宇宙中与水中微观宇宙相比始终更高 (至少是两倍).
- 土壤菌体隔离物呈现的衰变常数大约是以前报告的水生菌体的四倍.
结论:
- 土壤中菌体衰变的速度较慢表明营养率较低,可能会影响病毒介导的死亡率和细菌活动.
- 腐烂速率的广泛范围和有限的数据凸显了对土壤菌体生态和病毒宿主动态的进一步研究的需要.
相关概念视频
Viral Replication: Lysogenic Cycle
83
The lysogenic cycle is a crucial viral replication strategy that allows bacteriophages to persist within host cells without immediately destroying them. This process is primarily observed in temperate phages, such as bacteriophage lambda (λ), which infects Escherichia coli. The cycle allows the viral genome to persist across bacterial generations while keeping host cells viable.Integration of the Viral GenomeUpon infection, bacteriophage lambda attaches to the bacterial surface and injects...
83
Lytic Cycle of Bacteriophages
70.9K
Bacteriophages, also known as phages, are specialized viruses that infect bacteria. A key characteristic of phages is their distinctive “head-tail” morphology. A phage begins the infection process (i.e., lytic cycle) by attaching to the outside of a bacterial cell. Attachment is accomplished via proteins in the phage tail that bind to specific receptor proteins on the outer surface of the bacterium. The tail injects the phage’s DNA genome into the bacterial cytoplasm. In the...
70.9K
Lysogenic Cycle of Bacteriophages
62.4K
In contrast to the lytic cycle, phages infecting bacteria via the lysogenic cycle do not immediately kill their host cell. Instead, they combine their genome with the host genome, allowing the bacteria to replicate the phage DNA along with the bacterial genome. The incorporated copy of the phage genome is called the prophage. Some prophages can re-activate and enter the lytic cycle. This often occurs in response to a perturbation, such as DNA damage, but can also transpire in the absence of...
62.4K
Viral Replication: Lytic Cycle
85
Bacteriophages, or phages, are viruses that specifically infect bacteria. Among them, T-even bacteriophages, such as T4, exhibit a well-characterized lytic replication cycle in Escherichia coli (E. coli). This process ensures the rapid proliferation of the virus while ultimately leading to the destruction of the bacterial host.Attachment and DNA InjectionThe infection process begins with the recognition and binding of the T4 phage to the E. coli cell surface. Tail fibers of the phage...
85
Viruses of Archaea
31
Archaeal viruses play a crucial role in the ecosystems of extremophilic archaea, particularly those belonging to the phyla Euryarchaeota and Crenarchaeota. By shaping host evolution and facilitating gene transfer, these viruses influence microbial communities and contribute to genetic diversity in extreme environments. The archaea they infect thrive in acidic hot springs and hydrothermal vents characterized by high temperatures and low pH. Archaeal viruses exhibit remarkable structural...
31
DNA Bacteriophages
57
Bacteriophages, or phages, are viruses that specifically infect bacteria, utilizing their genetic material to hijack host cellular machinery for replication. DNA bacteriophages employ single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA) genomes. These phages exhibit diverse replication strategies and host interactions, influencing their ecological roles and applications in biotechnology and medicine.ssDNA BacteriophagesssDNA phages, with their small genomes, utilize unique strategies to...
57


