通过Bacillus subtilis使用阶段控制发酵和粘度降低策略增强聚-γ-胺酸的生产
Yin Guo1, Yuanyuan Liu1, Zejian Yang1
1State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi Microorganism and Enzyme Research Center of Engineering Technology, College of Life Science and Technology, Guangxi University, 100 Daxue Road, Nanning, 530004, Guangxi, China.
Applied biochemistry and biotechnology
|July 11, 2023
概括
这项研究优化了Bacillus subtilis通过使用双阶段受控发酵 (TSCF) 和降低粘度) 优化了聚-γ-胺酸 (PGA) 的产生. 结合这些策略,与非阶段控制发酵 (NSCF) 相比,PGA产量高达32.94%显著增加.
科学领域:
- 生物技术是生物技术.
- 微生物发酵 微生物发酵
- 生物化学工程 生物化学工程
背景情况:
- 聚-γ-胺酸 (PGA) 是一种多功能生物聚合物,具有众多应用.
- 细菌细菌是工业PGA生产的关键微生物.
- 高粘度和低溶解氧度限制PGA在常规发酵中的产量.
研究的目的:
- 为了研究和优化由 Bacillus subtilis.通过聚-γ-胺酸 (PGA) 生产.
- 开发和评估一个双阶段的受控发酵 (TSCF) 战略.
- 评估粘度降低对PGA发酵效率的影响.
主要方法:
- 为了确定最佳的发酵参数,进行了单因素优化实验.
- 实施了双阶段控制发酵 (TSCF),具体时间点为温度,pH,通风和.
- 粘度降低策略与TSCF集成,以解决高粘度的局限性.
主要成果:
- 与非阶段控制发酵 (NSCF) 相比,单独的TSCF并没有显著改善PGA标位.
- 结合TSCF和降低粘度,与NSCF相比,PGA标位增加了17.66%32.94%.
- 优化过程实现了PGA标位在25.00~30.67g/L之间.
结论:
- 结合TSCF和降低粘度的战略是有效的增强PGA生产在Bacillus subtilis.
- 这种方法为高粘度发酵系统提供了有价值的过程控制策略.
- 这些发现为优化工业生物聚合物生产提供了一个参考.
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