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Updated: Jul 23, 2025

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
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Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

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使用直接RNA测序技术在人体细胞中映射替代多基化

Mareike Polenkowski1, Aldrige Bernardus Allister2, Sebastian Burbano de Lara3

  • 1Department of Gastroenterology, Hepatology, Infectious Diseases and Endocrinology, Hannover Medical School, 30623 Hannover, Germany.

STAR protocols
|July 11, 2023
PubMed
概括

本研究提出了一种使用直接RNA测序的全基因组检测替代裂变和多化 (APA) 的协议. 该方法可以识别具有不同3'未翻译区域的mRNA异型.

关键词:
基因表达 基因表达序列分析 序列分析

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RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
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