通过使用集成图书馆指导分析,对尿中的美卡普酸进行全球分析
Zhengzhi Xie1,2,3,4, Jin Y Chen1,2,3,4, Hong Gao1,2,3,4
1American Heart Association-Tobacco Regulation and Addiction Center, University of Louisville, Louisville, Kentucky 40202, United States.
Environmental science & technology
|July 11, 2023
概括
这项研究引入了一种新的方法来识别超过100种尿液中的酸 (MAs),包括一些新型的酸,这些酸在吸烟者体内含量较高,原因是环境中有毒物质.
科学领域:
- 环境化学环境化学
- 生物标志物发现发现
- 分析化学 分析化学
背景情况:
- 尿液中的默卡普特里酸 (MAs) 是评估人类暴露于环境和职业异生菌的关键生物标志物.
- 以前用于MA分析的非定向方法在范围和准确性方面存在限制.
研究的目的:
- 开发和验证一个先进的图书馆指导的工作流程,用于全面的尿道MA概况.
- 在人体尿样中识别和量化已知和新型MAs.
主要方法:
- 使用超高性能液态染色学四极飞行时间质谱法 (UHPLC-QTOF-MS).
- 开发了一个集成的工作流程,扩展了分配标准,并策划了220个MA的库.
- 分析了来自70名参与者的尿样 (40名非吸烟者,30名吸烟者).
主要成果:
- 假定注释了来自63种前体的116种MA,其中包括25种以前未报告的MA.
- 确定了每个样本大约500个MA候选者.
- 在吸烟者中发现了46MAs的显著升高水平,与香烟烟雾中的有毒物质如乙烯烯和有关.
结论:
- 开发的工作流程能够从多种来源对已知和新型MA进行可靠的分析.
- 吸烟者表现出较高水平的特定MA,表明对某些环境有毒物质的暴露较高.
- 该方法可以适应更广泛的暴露范围的关联研究.
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