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一个卵巢表型的alpha 7尼古丁受体淘汰赛小鼠

Pia Seßenhausen¹, Karolina M Caban², Nicole Kreitmair¹

¹Biomedical Center Munich (BMC), Cell Biology, Anatomy III, Faculty of Medicine, Ludwig Maximilian University of Munich, Planegg-Martinsried, Germany.

Reproduction (Cambridge, England)

|July 11, 2023

概括

尼古丁乙胆受体α7 (nAChRa7) 在卵巢功能中起作用. 在小鼠中缺乏nAChRa7会改变卵巢肌瘤细胞和小卵泡数量,这表明它参与了卵巢局部调节.

科学领域:

生殖生物学生殖生物学
神经内分泌学神经内分泌学
分子生物学分子生物学

背景情况:

尼古丁乙胆受体α7 (nAChRa7) 在各种细胞中表达,并参与多种细胞功能.
nAChRa7存在于成年小鼠的卵巢中,可能存在于细胞,巨细胞和卵细胞中.

研究的目的:

为了研究nAChRa7在卵巢功能中的作用,使用Chrna7淘汰赛 (KO) 小鼠.
评估nAChRa7缺失对卵巢形态,细胞群和蛋白质表达的影响.

主要方法:

对Chrna7 KO和野生型 (WT) 成年小鼠卵巢进行比较分析.
使用免疫组织化学,qPCR,血清孕激素测量和蛋白质组分析.
评估了卵巢形态,卵泡数量,缩和蛋白质丰度.

主要成果:

与WT相比,Chrna7 KO小鼠的原始毛囊数量较少,但其他毛囊类型和白色体的数量相似.
没有观察到血清孕激素或大多数增殖/亡标志物的显著变化.
蛋白质组分析显示了蛋白质丰度的显著变化,在KO卵巢中增加了肌肉细胞和巨细胞的标记物.

结论:

缺少nAChRa7会导致卵巢干细胞种群的变化,并影响小毛囊数量.
这些发现将nAChRa7与卵巢细胞的局部调节联系起来,特别是肌体细胞.

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