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电刺激诱导的结构性3D人造神经组织,具有发达的神经网络和功能连接

Xiaoting Meng¹, Xiyao Yu¹, Yingli Lu²

¹Department of Histology & Embryology, College of Basic Medical Sciences, Jilin University, Changchun 130021, People's Republic of China.

Journal of neural engineering

|July 11, 2023

概括

电刺激 (ES) 成功创建了有组织的3D人造神经组织 (hENT). 这项研究证明了ES诱导的hENT的结构和功能特性,为神经疾病模型和中枢神经系统损伤治疗铺平了道路.

科学领域:

神经科学是一个神经科学.
生物技术是生物技术.
组织工程是组织工程.

背景情况:

三维 (3D) 神经组织工程为神经疾病建模和中枢神经系统 (CNS) 损伤修复提供了前景.
之前的工作建立了一个电刺激 (ES) 系统用于小鼠工程神经组织 (mENT) in vitro.
由ES诱导的人类工程神经组织 (hENT) 的结构和功能特征仍然未被探索.

研究的目的:

研究ES在3D Matrigel环境中调节人类神经干细胞的应用.
探索ES诱导的hENT的结构部件和功能性质.
评估hENT在神经疾病建模和中枢神经系统损伤治疗方面的潜力.

主要方法:

免疫光染色和电子显微镜被用于评估hENT中的神经元分化,神经元外生,突触形成和髓膜发育.
使用成像检测神经元活动和hENT中的电刺激性.
进行了老鼠和人类组织的ex vivo融合,以调查突触连接.

主要成果:

在hENT中,ES有效诱导了神经元分化,组织了神经元生长和神经元亚型的成熟.
一个发达的神经元网络与突触和髓膜被构建.
细胞外K+度增加导致神经元刺激能力提高,证实了hENT中的电活动.

结论:

电刺激是一种可行的方法,用于生成有组织的3D人体工程神经组织 (hENT).
由ES诱导的hENT表现出神经组织的结构和功能特征.
这些发现支持hENT在开发高级神经疾病模型和中枢神经系统损伤治疗策略方面的潜力.

关键词:

工程神经组织 (ENT) 的工程神经组织.人类神经干细胞的神经干细胞神经元分化神经元的分化神经网络的神经网络的神经网络生理学电场是一种生理学电场.

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