Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Preparation of Samples for Electron Microscopy01:20

Preparation of Samples for Electron Microscopy

5.5K
To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...
5.5K
X-ray Diffraction of Biological Samples01:10

X-ray Diffraction of Biological Samples

3.9K
X-ray diffraction or XRD is an analytical tool that utilizes X-rays to study ordered structures such as crystalline organic and inorganic samples, polycrystalline materials, proteins, carbohydrates, and drugs.
According to Bragg's law, when X-rays strike the sample positioned on a stage, the rays are  scattered by the electron clouds around the sample atoms. The  X-ray diffraction or scattering is caused by constructive interference of the X-ray waves that reflect off the internal...
3.9K
Dehydration Synthesis01:15

Dehydration Synthesis

133.7K
Overview
Dehydration synthesis (also called a condensation reaction) is the chemical process in which two molecules covalently link together to form a new molecule, along with the release of a water molecule. Many physiologically important compounds form by dehydration synthesis reactions, such as complex carbohydrates, proteins, DNA, and RNA.
Synthesis of carbohydrates
Sugar molecules are covalently linked together by dehydration synthesis. During the reaction, the hydroxyl (-OH) group from...
133.7K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Cumulative traumatic life events and increased risk for emergency department and inpatient utilization after physical injury hospitalization.

Injury·2026
Same author

Coronary Artery Calcium Score Predicts Cardiovascular and Transplant-Related Outcomes in Lung Transplant Recipients.

Clinical transplantation·2026
Same author

Interior soft x-ray tomography with sparse global sampling.

Physica scripta·2026
Same author

Paroxysmal atrial fibrillation-like bursts in racehorses: do they affect speed and performance? A case control study.

Journal of veterinary internal medicine·2026
Same author

Common TF-IDF variants arise as key components in the test statistic of a penalized likelihood-ratio test for word burstiness.

Discover computing·2026
Same author

REPLY.

Ophthalmology·2026

相关实验视频

Updated: Jul 23, 2025

3D Imaging of Soft-Tissue Samples using an X-ray Specific Staining Method and Nanoscopic Computed Tomography
07:01

3D Imaging of Soft-Tissue Samples using an X-ray Specific Staining Method and Nanoscopic Computed Tomography

Published on: October 24, 2019

9.9K

脱水作为软X射线断层扫描的替代样品制备方法.

Anthoula Chatzimpinou1, Charlotta Funaya2, David Rogers3

  • 1Centre for Organismal Studies (COS), Heidelberg University, Heidelberg, Germany.

Journal of microscopy
|July 11, 2023
PubMed
概括

软X射线断层扫描 (SXT) 可以通过临界点干燥 (CPD) 在不结的情况下成像细胞. 用CPD准备的细胞提供了与冷保存样品相比较的超结构细节,使SXT在没有冷设备的情况下可访问.

关键词:
在X射线中吸收的X射线吸收.细胞解剖学 细胞解剖学脱水 脱水 脱水 脱水断层扫描 (tomography) 是一个非常重要的技术.

更多相关视频

A 3D Cartographic Description of the Cell by Cryo Soft X-ray Tomography
08:47

A 3D Cartographic Description of the Cell by Cryo Soft X-ray Tomography

Published on: March 15, 2021

4.0K
Cell Culture on Silicon Nitride Membranes and Cryopreparation for Synchrotron X-ray Fluorescence Nano-analysis
08:26

Cell Culture on Silicon Nitride Membranes and Cryopreparation for Synchrotron X-ray Fluorescence Nano-analysis

Published on: December 10, 2019

9.3K

相关实验视频

Last Updated: Jul 23, 2025

3D Imaging of Soft-Tissue Samples using an X-ray Specific Staining Method and Nanoscopic Computed Tomography
07:01

3D Imaging of Soft-Tissue Samples using an X-ray Specific Staining Method and Nanoscopic Computed Tomography

Published on: October 24, 2019

9.9K
A 3D Cartographic Description of the Cell by Cryo Soft X-ray Tomography
08:47

A 3D Cartographic Description of the Cell by Cryo Soft X-ray Tomography

Published on: March 15, 2021

4.0K
Cell Culture on Silicon Nitride Membranes and Cryopreparation for Synchrotron X-ray Fluorescence Nano-analysis
08:26

Cell Culture on Silicon Nitride Membranes and Cryopreparation for Synchrotron X-ray Fluorescence Nano-analysis

Published on: December 10, 2019

9.3K

科学领域:

  • 细胞生物学 细胞生物学
  • 显微镜的使用方法
  • 生物物理学的生物物理.

背景情况:

  • 软X射线断层扫描 (SXT) 能够在近原生状态下对整个细胞进行高分辨率成像.
  • 目前的SXT协议需要冷保存,由于特殊设备需求,限制了可访问性.
  • 寻求替代样品制备方法以扩大SXT的适用性.

研究的目的:

  • 在脱水细胞样本上研究SXT成像的可行性.
  • 为了比较不同的脱水技术用于超结构性保存和收缩.
  • 建立关键点干燥 (CPD) 作为一个可行的SXT样品准备方法.

主要方法:

  • 在小鼠胚胎纤维细胞上对各种脱水过程 (包括临界点干燥和空气干燥) 的比较.
  • 评估脱水后的超结构性保存和细胞收缩.
  • 冷保存,空气干燥和CPD干燥细胞的软X射线断层成像.
  • 对脱水细胞的X射线吸收和3D解剖细分的分析.

主要成果:

  • 与空气干燥相比,临界点干燥 (CPD) 证明了优越的超结构性保存.
  • 经过CPD干燥的细胞表现出高的结构完整性,最小的收缩.
  • 虽然CPD干细胞显示有机细胞的X射线吸收增加 (3-7x),但差异吸收保持不变.
  • 通过CPD干燥细胞的3D解剖学可以成功地被细分和分析.

结论:

  • 脱水,特别是临界点干燥 (CPD),是一种可行的替代样本准备方法,用于软X射线断层扫描 (SXT).
  • CPD允许使用SXT对细胞进行超结构可视化,而不需要冷保存.
  • 这种方法将SXT成像的可访问性扩展到缺乏冷基础设施的实验室.