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在Pvr4中由α1-2螺旋体控制的蛋白质稳定性对于局部化和细胞死亡至关重要

Hye-Young Lee¹, Jeen Choi^1,2, Minji Kang^1,2

¹Plant Immunity Research Center, Seoul National University, Seoul, 08826, Republic of Korea.

The Plant journal : for cell and molecular biology

|July 11, 2023

概括

植物免疫受体使用CC域来发送信号. 这项研究确定了CC^Pvr4中的关键残留物,这些残留物对于稳定性,血向和细胞死亡信号至关重要,揭示了植物疾病耐药性的机制.

科学领域:

植物免疫力植物免疫力
分子植物病理学分子植物病理学
细胞信号传递细胞信号传递

背景情况:

核酸结合域氨酸丰富重复受体 (NLR) 蛋白质通过识别病原体作用因子,赋予植物疾病耐药性.
虽然NLRs的CC域与免疫信号传递和细胞死亡诱导有关,但其确切的机制尚不清楚.
抗病毒NLR蛋白Pvr4 (CC^Pvr4) 的CC域在过度表达时诱导细胞死亡.

研究的目的:

阐明CC^Pvr4介导的细胞死亡的分子机制.
在CC^Pvr4中识别关键的残留物和结构特征,负责其功能.

主要方法:

使用易发生错误的PCR基于随机突变发生的功能丧失CC^Pvr4突变的产生.
细胞生物学和生物化学分析以评估蛋白质稳定性,血局部化,寡合化和相互作用.
绿色光蛋白 (GFP) 标记,以评估蛋白质稳定性对功能的影响.

主要成果:

在M16和Q52的突变破坏了CC^Pvr4稳定性,血局部化和寡合化,但可以通过GFP标记恢复稳定性,重新建立细胞死亡活动.
在N端区域的一个突变 (I7E) 通过削弱与血膜H⁺-ATPase的相互作用而失去诱导细胞死亡的活动,尽管保持了血膜局部化.
分析表明,无序的N端区域对于血关联和向至关重要.

结论:

特定残留物 (M16,Q52) 和CC^Pvr4的N端区域对于其稳定性,局部化和诱导细胞死亡的活动至关重要.
CC^Pvr4功能涉及到等离子体膜的关联和与H⁺-ATPase.等组件的相互作用.
这项研究提供了关于植物NLR免疫受体的信号机制的见解.

关键词:

CC域名 CC域名 CC域名 CC域名 Pvr4vr4 在线观看细胞死亡是细胞死亡.氧化聚合物的一种.植物的NLR是植物的NLR.血膜中的H+-ATPase酶

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