同时对六种植物来源的中国蜂蜜中的挥发性成分进行定性和定量分析,使用头部空间固相微提取和气相色谱-质谱仪
Dongshuang Liang1,2, Haosong Wen2, Yaxuan Zhou2
1Hebei Key Laboratory of Applied Chemistry, College of Environmental and Chemical Engineering, Yanshan University, Qinhuangdao, China.
Journal of the science of food and agriculture
|July 11, 2023
概括
一种新的头部空间固态微提取和气色谱质谱法 (HS-SPME-GC-MS) 方法可以准确地分析蜂蜜.
科学领域:
- 分析化学 分析化学
- 食品科学 食品科学 食品科学
- 有机化学 有机化学
背景情况:
- 蜂蜜香味是主要特征,受挥发性化合物的影响.
- 分析挥发性资料对于蜂蜜的真实性和确定植物来源至关重要.
- 以前的方法缺乏对多种挥发性化合物的同时定性和定量分析.
研究的目的:
- 开发和验证一种HS-SPME-GC-MS方法,用于分析蜂蜜中的挥发性化合物.
- 在六种不同的蜂蜜类型中同时识别和量化34种挥发性成分.
- 为了确定蜂蜜真实性的挥发性指纹.
主要方法:
- 头部空间固态微提取 (HS-SPME) 用于样品制备.
- 气色谱-质谱 (GC-MS) 用SCAN+SIM模式进行分析.
- 化学方法 (主要成分分析,OPLS-DA) 用于分类.
主要成果:
- 开发的HS-SPME-GC-MS方法实现了高灵敏度的LOQ和LOD在ng/g范围内.
- 精确的量化,恢复率在70.6%至126.2%之间,RSD低 (≤45.4%).
- 根据它们的挥发性概况成功分类了六种单花蜂蜜类型.
结论:
- HS-SPME-GC-MS方法对于挥发性指纹和蜂蜜的定量分析是有效的.
- 确定了蜂蜜类型及其挥发性化合物组成之间的显著相关性.
- 该研究提供了一种强大的方法,用于蜂蜜认证和特征单花蜂蜜挥发物.
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