相关实验视频
Updated: Jul 23, 2025

10:36
Measurements of Physiological Stress Responses in C. Elegans
Published on: May 21, 2020
14.0K
一种RNA修饰酶直接感知活性氧物种,以调节Enterococcus faecalis中的转化
Wei Lin Lee1, Ameya Sinha1,2,3, Ling Ning Lam2,4,5
1Antimicrobial Resistance IRG, Singapore MIT Alliance for Research and Technology, Singapore, Singapore.
Nature communications
|July 11, 2023
概括
研究人员在细菌中发现了一种新的RNA修饰酶RlmN,它可以作为一个开关. 它感知到活性氧物种 (ROS) 并控制氧化应激期间的蛋白质翻译.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 细菌利用复杂的系统来抵消来自免疫反应和环境因素的反应性氧和物种 (ROS).
- 细菌应激反应的调节对于生存和病变发生至关重要.
研究的目的:
- 确定和描述细菌感知和响应分子层面的氧化应激的新机制.
- 研究RNA修饰在细菌适应环境挑战中的作用.
主要方法:
- 在氧化应激条件下对*Enterococcus faecalis*的tRNA表写体的分析.
- 研究甲基转移酶RlmN在对活性氧物种 (ROS) 的反应中的活性.
- 对E. faecalis进行蛋白质组分析,对RlmN酶进行基因淘汰.
主要成果:
- 在ROS暴露下,确定了核糖体RNA (rRNA) 和转移RNA (tRNA) 中N2 - - 甲基氨酸 (m2A) 的显著降低.
- 确定ROS使含有Fe-S集群的甲基转移酶RlmN失活,导致观察到的m2A的减少.
- 证明RlmN淘汰导致一种类似氧化应激的蛋白质组,具有增加的超氧化物转化酶和减少的毒性蛋白质.
结论:
- RlmN 作为一个氧化还原敏感分子开关,通过rRNA和tRNA修改直接将氧化应激检测与转化控制联系起来.
- 这项研究揭示了一种新的,对环境有反应的rRNA修饰动态,扩大了RNA修饰在直接蛋白质组调节中的范式.
- 这些发现为细菌适应策略和治疗干预的潜在目标提供了新的见解.
相关概念视频
Translational Regulation
45
Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
45
Protein Modifications in the RER
5.3K
Modification of secretory and transmembrane proteins entering the rough ER begins in the ER lumen. These modifications aid in protein folding and stabilize the acquired tertiary structure. Protein modifications in the rough ER co-occur at different stages of protein folding.
Broadly, these modifications can be categorized into four main categories — glycosylation, formation of disulfide bonds, assembly of protein subunits, and specific proteolytic cleavages like removal of signal...
Broadly, these modifications can be categorized into four main categories — glycosylation, formation of disulfide bonds, assembly of protein subunits, and specific proteolytic cleavages like removal of signal...
5.3K
Stringent Response in E. coli
31
Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...
31
Regulation of the Unfolded Protein Response
2.5K
Inositol-requiring kinase one or IRE1 is the most conserved eukaryotic unfolded protein response (UPR) receptor. It is a type I transmembrane protein kinase receptor with a distinctive site-specific RNase activity. As the binding mechanics of the misfolded proteins with the N-terminal domain of IRE-1 are unclear, three binding models — direct, indirect, and allosteric -- are proposed for receptor activation. Nevertheless, it is known that once a misfolded protein associates with IRE1, it...
2.5K
Transcriptional Regulation: Riboswitches
61
Riboswitches are RNA elements that regulate gene expression by altering their secondary structures in response to specific effector molecules. These elements, located in the leader regions of certain mRNAs, act as transcriptional regulators by toggling between alternative conformations to control downstream gene expression. Riboswitch-mediated regulation is a precise mechanism for modulating biosynthetic pathways, as exemplified by the riboflavin biosynthesis pathway in Bacillus...
61
Types of RNA
64.0K
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
64.0K

